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TSPAN31 Polyclonal antibody

TSPAN31 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 21987-1-AP

Print datasheet

Synonyms

Sarcoma amplified sequence, SAS, tetraspanin 31, Tspan 31, TSPAN31

验证数据展示

  • WB analysis of HeLa using 21987-1-AP

    WB analysis of HeLa using 21987-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21987-1-AP (TSPAN31 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 21987-1-AP

    IHC staining of human skeletal muscle using 21987-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21987-1-AP (TSPAN31 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human skeletal muscle using 21987-1-AP

    IHC staining of human skeletal muscle using 21987-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21987-1-AP (TSPAN31 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, mouse skeletal muscle tissue, PC-3 cells
Positive IHC detected inhuman skeletal muscle tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

The immunogen of 21987-1-AP is TSPAN31 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Cited ApplicationsIHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen TSPAN31 fusion protein Ag17138 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tetraspanin 31
Synonyms Sarcoma amplified sequence, SAS, tetraspanin 31, Tspan 31, TSPAN31
Calculated Molecular Weight 210 aa, 23 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC010377
Gene Symbol TSPAN31
Gene ID (NCBI) 6302
RRIDAB_2878962
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12999
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tetraspanin-31 (TSPAN31) belongs to the tetraspanin (TM4SF) family, also known as the transmembrane 4 (TM4) superfamily, which are a family of transmembrane proteins with 33 mammalian members. These proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. Tetraspanin-31 is thought to be involved in growth-related cellular processes. The experimentally determined molecular mass of 30 kDa is larger than the calculated molecular mass of 23 kDa, which could be a result of post-translational modifications.

实验方案

Product Specific Protocols
WB protocol for TSPAN31 antibody 21987-1-APDownload protocol
IHC protocol for TSPAN31 antibody 21987-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cancer Sci

Overexpression of Tetraspanin31 contributes to malignant potential and poor outcomes in gastric cancer

Authors - Yusuke Takashima
View Article