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TSPAN2 Polyclonal antibody

TSPAN2 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 20463-1-AP

Print datasheet

Synonyms

6330415F13Rik, NET3, Tetraspan NET 3, tetraspanin 2, TSN2, TSPAN 2, TSPAN2

验证数据展示

  • WB analysis using 20463-1-AP

    WB analysis using 20463-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20463-1-AP (TSPAN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HL-60 using 20463-1-AP

    IP experiment of HL-60 using 20463-1-AP

    IP result of anti-TSPAN2 (IP:20463-1-AP, 4ug; Detection:20463-1-AP 1:500) with HL-60 cells lysate 960ug.

经过测试的应用

Positive WB detected inHL-60 cells, K-562 cells
Positive IP detected inHL-60 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20463-1-AP targets TSPAN2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen TSPAN2 fusion protein Ag14299 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tetraspanin 2
Synonyms 6330415F13Rik, NET3, Tetraspan NET 3, tetraspanin 2, TSN2, TSPAN 2, TSPAN2
Calculated Molecular Weight 221 aa, 24 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC021675
Gene Symbol TSPAN2
Gene ID (NCBI) 10100
RRIDAB_2878691
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60636
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSPAN2 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. It has been reported that TSPAN2 is involved in cell invasion and motility during lung cancer progression (PMID: 24726368). TSPAN2 enhances cell motility and invasiveness by assisting CD44 in scavenging intracellular reactive oxygen species.

实验方案

Product Specific Protocols
WB protocol for TSPAN2 antibody 20463-1-APDownload protocol
IP protocol for TSPAN2 antibody 20463-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Elife

Conservation and divergence of myelin proteome and oligodendrocyte transcriptome profiles between humans and mice.

Authors - Vasiliki-Ilya Gargareta
View Article
humanIF

FASEB J

Selective expression of TSPAN2 in vascular smooth muscle is independently regulated by TGF-β1/SMAD and myocardin/serum response factor.

Authors - Jinjing Zhao
View Article