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TSHR Polyclonal antibody

TSHR Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14450-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, ELISA and More (2)

TSH R, Thyrotropin receptor, Thyroid-stimulating hormone receptor, LGR3, hTSHR I

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inTT cells, HeLa cells, mouse ovary tissue, mouse thymus tissue
Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14450-1-AP targets TSHR in WB, IHC, IF, ELISA, ICC-IF applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF, ICC-IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, zebrafish
免疫原 TSHR fusion protein Ag5957 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 thyroid stimulating hormone receptor
别名 TSH R, Thyrotropin receptor, Thyroid-stimulating hormone receptor, LGR3, hTSHR I
计算分子量 87 kDa
观测分子量87kDa, 62-70kDa, 28-30kDa
GenBank蛋白编号BC063613
基因名称 TSHR
Gene ID (NCBI) 7253
RRIDAB_2208263
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16473
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Thyroid stimulating hormone (also known as thyrotropin or TSH) is a glycoprotein hormone produced by thyrotrophs in the anterior pituitary gland. The TSH receptor (TSHR), mainly expressed on the thyroid epithelial cells, is a G protein-coupled receptor (GPCR) of the seven-transmembrane domain family and plays a central role in controlling thyroid cell metabolism. The gene of TSHR maps to chromosome 14q31.1, and encodes a 764-amino acid protein with a predicted unmodified molecular weight of 86.8 kDa. The apparent molecular weight of glycosylated form is 95-120 kDa. Studies reveal that TSHR is composed of two subunits, A and B, which are produced by cleavage of single-chain TSHR on the cell surface and subsequently connected by disulfide bonds. This rabbit polyclonal antibody raised against N-terminal region (1-253 aa) of human TSHR can detect glycosylated subunit A with an experimentally determined molecular weight of 62-70 kDa under reducing condition. (PMID: 20578897, 17158770, 12593718)

实验方案

Product Specific Protocols
WB protocol for TSHR antibody 14450-1-APDownload protocol
IHC protocol for TSHR antibody 14450-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Theranostics

Obatoclax and LY3009120 Efficiently Overcome Vemurafenib Resistance in Differentiated Thyroid Cancer.

Authors - Wei-Jun Wei
humanIF

Int J Nanomedicine

A TSHR-Targeting Aptamer in Monocytes Correlating with Clinical Activity in TAO

Authors - Jiamin Cao
mouseIHC

Oxid Med Cell Longev

TSH Combined with TSHR Aggravates Diabetic Peripheral Neuropathy by Promoting Oxidative Stress and Apoptosis in Schwann Cells.

Authors - Jingwen Fan
zebrafishWB

Environ Pollut

Acute exposure of zebrafish embryo (Danio rerio) to flutolanil reveals its developmental mechanism of toxicity via disrupting the thyroid system and metabolism.

Authors - Miaomiao Teng
humanIF

Cancers (Basel)

Patient-Derived Papillary Thyroid Cancer Organoids for Radioactive Iodine Refractory Screening.

Authors - Luc H J Sondorp
humanWB

Front Endocrinol (Lausanne)

Long Non-coding Antisense RNA TNRC6C-AS1 Is Activated in Papillary Thyroid Cancer and Promotes Cancer Progression by Suppressing TNRC6C Expression.

Authors - Dilidaer Muhanhali
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