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验证数据展示

  • IHC staining of human pituitary using 66750-1-Ig

    IHC staining of human pituitary using 66750-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66750-1-Ig (TSH beta antibody) at dilution of 1:6000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human pituitary using 66750-1-Ig

    IF Staining of human pituitary using 66750-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human pituitary tissue using TSH Beta antibody (66750-1-Ig, Clone: 2G1H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IHC staining of human pituitary using 66750-1-Ig

    IHC staining of human pituitary using 66750-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66750-1-Ig (TSH beta antibody) at dilution of 1:6000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human pituitary using 66750-1-Ig

    IF Staining of human pituitary using 66750-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human pituitary tissue using TSH Beta antibody (66750-1-Ig, Clone: 2G1H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

TSH Beta Monoclonal antibody

TSH Beta Monoclonal Antibody for IHC, IF-P, ELISA
Cat No. 66750-1-Ig

产品说明书

CloneNo. 2G1H3
引用文献(1)

宿主/亚型

Mouse / IgG2b

种属反应性

Human and More (1)

应用

IHC, IF-P, ELISA and More (1)

别名

CHNG4, Thyrotropin alfa, Thyrotropin beta chain, Thyrotropin subunit beta, TSH B, TSH BETA, TSHB

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman pituitary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman pituitary tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:3000-1:12000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66750-1-Ig targets TSH Beta in IHC, IF-P, ELISA applications and shows reactivity with Human samples.

经测试应用 IHC, IF-P, ELISA Application Description
文献引用应用IHC, IF
经测试反应性 Human
文献引用反应性human
免疫原 TSH Beta fusion protein Ag27153 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 thyroid stimulating hormone, beta
别名 CHNG4, Thyrotropin alfa, Thyrotropin beta chain, Thyrotropin subunit beta, TSH B, TSH BETA, TSHB
计算分子量 138 aa, 16 kDa
GenBank蛋白编号BC069298
基因名称 TSH beta
Gene ID (NCBI) 7252
RRIDAB_2882097
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01222
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for TSH Beta antibody 66750-1-IgDownload protocol
IF protocol for TSH Beta antibody 66750-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,IF

Cancer Commun (Lond)

Local TSH/TSHR signaling promotes CD8+ T cell exhaustion and immune evasion in colorectal carcinoma

Authors - Sisi Zeng
View Article