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验证数据展示

  • WB analysis using 17566-1-AP

    WB analysis using 17566-1-AP

    HEK-293 cell lysates were subjected to SDS PAGE followed by western blot with 17566-1-AP (TSGA14 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of rat testis using 17566-1-AP

    IHC staining of rat testis using 17566-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17566-1-AP (TSGA14 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

TSGA14 Polyclonal antibody

TSGA14 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17566-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Centrosomal protein of 41 kDa, Cep41, testis specific, 14, Testis-specific gene A14 protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inrat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17566-1-AP targets TSGA14 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原

CatNo: Ag11764

Product name: Recombinant human TSGA14 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-301 aa of BC056162

Sequence: MSLRRHIGNPEYLMKRIPQNPRYQHIKSRLDTGNSMTKYTEKLEEIKKNYRYKKDELFKRLKVTTFAQLIIQVASLSDQTLEVTAEEIQRLEDNDSAASDPDAETTARTNGKGNPGEQSPSPEQFINNAGAGDSSRSTLQSVISGVGELDLDKGPVKKAEPHTKDKPYPDCPFLLLDVRDRDSYQQCHIVGAYSYPIATLSRTMNPYSNDILEYKNAHGKIIILYDDDERLASQAATTMCERGFENLFMLSGGRLNQANSSGRESKVPGARSAQNLPGGGPASHSNPRSLSSGHLQGKPWK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 testis specific, 14
别名 Centrosomal protein of 41 kDa, Cep41, testis specific, 14, Testis-specific gene A14 protein
计算分子量301aa,33 kDa; 373aa,41 kDa
观测分子量 45 kDa
GenBank蛋白编号BC056162
基因名称 TSGA14
Gene ID (NCBI) 95681
RRIDAB_2878417
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BYV8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSGA14 (testis-specific Gene A14), also known as CEP41 (centrosomal protein of 41 kDa) , is a centrosomal protein identified in a mass-spectrometry-based proteomic analysis of human centrosomes. Mutations in the TSGA14 gene have been identified in families with autism spectrum disorders. (PMID:21438139)

实验方案

Product Specific Protocols
WB protocol for TSGA14 antibody 17566-1-APDownload protocol
IHC protocol for TSGA14 antibody 17566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Cell Sci

CEP41, a ciliopathy-linked centrosomal protein, regulates microtubule assembly and cell proliferation

Authors - Shweta Shyam Prassanawar
  • KD Validated
View Article