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验证数据展示

  • WB analysis using 17566-1-AP

    WB analysis using 17566-1-AP

    HEK-293 cell lysates were subjected to SDS PAGE followed by western blot with 17566-1-AP (TSGA14 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of rat testis using 17566-1-AP

    IHC staining of rat testis using 17566-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17566-1-AP (TSGA14 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

TSGA14 Polyclonal antibody

TSGA14 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17566-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Centrosomal protein of 41 kDa, Cep41, testis specific, 14, Testis-specific gene A14 protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inrat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17566-1-AP targets TSGA14 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 TSGA14 fusion protein Ag11764 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 testis specific, 14
别名 Centrosomal protein of 41 kDa, Cep41, testis specific, 14, Testis-specific gene A14 protein
计算分子量301aa,33 kDa; 373aa,41 kDa
观测分子量 45 kDa
GenBank蛋白编号BC056162
基因名称 TSGA14
Gene ID (NCBI) 95681
RRIDAB_2878417
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BYV8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSGA14 (testis-specific Gene A14), also known as CEP41 (centrosomal protein of 41 kDa) , is a centrosomal protein identified in a mass-spectrometry-based proteomic analysis of human centrosomes. Mutations in the TSGA14 gene have been identified in families with autism spectrum disorders. (PMID:21438139)

实验方案

Product Specific Protocols
WB protocol for TSGA14 antibody 17566-1-APDownload protocol
IHC protocol for TSGA14 antibody 17566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Cell Sci

CEP41, a ciliopathy-linked centrosomal protein, regulates microtubule assembly and cell proliferation

Authors - Shweta Shyam Prassanawar
  • KD Validated
View Article