验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells |
| Positive IHC detected in | rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17566-1-AP targets CEP41 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag11764 Product name: Recombinant human TSGA14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-301 aa of BC056162 Sequence: MSLRRHIGNPEYLMKRIPQNPRYQHIKSRLDTGNSMTKYTEKLEEIKKNYRYKKDELFKRLKVTTFAQLIIQVASLSDQTLEVTAEEIQRLEDNDSAASDPDAETTARTNGKGNPGEQSPSPEQFINNAGAGDSSRSTLQSVISGVGELDLDKGPVKKAEPHTKDKPYPDCPFLLLDVRDRDSYQQCHIVGAYSYPIATLSRTMNPYSNDILEYKNAHGKIIILYDDDERLASQAATTMCERGFENLFMLSGGRLNQANSSGRESKVPGARSAQNLPGGGPASHSNPRSLSSGHLQGKPWK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | testis specific, 14 |
| 别名 | TSGA14, Centrosomal protein of 41 kDa, testis specific, 14, Testis-specific gene A14 protein |
| 计算分子量 | 301aa,33 kDa; 373aa,41 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC056162 |
| 基因名称 | TSGA14 |
| Gene ID (NCBI) | 95681 |
| RRID | AB_2878417 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BYV8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TSGA14 (testis-specific Gene A14), also known as CEP41 (centrosomal protein of 41 kDa) , is a centrosomal protein identified in a mass-spectrometry-based proteomic analysis of human centrosomes. Mutations in the TSGA14 gene have been identified in families with autism spectrum disorders. (PMID:21438139)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CEP41 antibody 17566-1-AP | Download protocol |
| WB protocol for CEP41 antibody 17566-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Sci CEP41, a ciliopathy-linked centrosomal protein, regulates microtubule assembly and cell proliferation
| ||
Mol Psychiatry ASD mutations in the ciliary gene CEP41 impact development of projection neurons and interneurons in a human cortical organoid model. |
