• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67381-1-Ig (same clone as 67381-1-PBS)

    WB analysis using 67381-1-Ig (same clone as 67381-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67381-1-Ig (TSG101 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 67381-1-Ig (same clone as 67381-1-PBS)

    IF Staining of human colon cancer using 67381-1-Ig (same clone as 67381-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using TSG101 antibody (67381-1-Ig, Clone: 2B7G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67381-1-Ig (same clone as 67381-1-PBS)

    IHC staining of human lung cancer using 67381-1-Ig (same clone as 67381-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67381-1-Ig (TSG101 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TSG101 antibody included
  • IF Staining of HeLa using 67381-1-Ig (same clone as 67381-1-PBS)

    IF Staining of HeLa using 67381-1-Ig (same clone as 67381-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67381-1-Ig (TSG101 antibody), at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

  • WB analysis of human urine exosomes using 67381-1-Ig (same clone as 67381-1-PBS)

    WB analysis of human urine exosomes using 67381-1-Ig (same clone as 67381-1-PBS)

    human urine exosomes tissue were subjected to SDS PAGE followed by western blot with 67381-1-Ig (TSG101 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 67381-1-Ig (same clone as 67381-1-PBS)

    FC experiment of HeLa using 67381-1-Ig (same clone as 67381-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TSG101 Monoclonal antibody (67381-1-Ig, Clone:2B7G8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

TSG101 Monoclonal antibody, PBS Only

TSG101 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Cat No. 67381-1-PBS

产品说明书

CloneNo. 2B7G8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

别名

2B7G8, ESCRT-I complex subunit TSG101, TSG 10, TSG 101, TSG10

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy TSG101 Ready-To-Use IHC Kit. TSG101 primary antibody included.

产品信息

67381-1-PBS targets TSG101 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag28569

Product name: Recombinant human TSG101 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 214-390 aa of BC002487

Sequence: GPSRDGTISEDTIRASLISAVSDKLRWRMKEEMDRAQAELNALKRTEEDLKKGHQKLEEMVTRLDQEVAEVDKNIELLKKKDEELSSALEKMENQSENNDIDEVIIPTAPLYKQILNLYAEENAIEDTILYLGEALRRGVIDLDVFLKHVRLLSRKQFQLRALMQKARKTAGLSDLY

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tumor susceptibility gene 101
别名 2B7G8, ESCRT-I complex subunit TSG101, TSG 10, TSG 101, TSG10
计算分子量 44 kDa
观测分子量 43-46 kDa
GenBank蛋白编号BC002487
基因名称 TSG101
Gene ID (NCBI) 7251
RRIDAB_2882628
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ99816
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.