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验证数据展示

  • WB analysis of HeLa using 11701-1-AP

    WB analysis of HeLa using 11701-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11701-1-AP (TSFM Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 11701-1-AP

    IHC staining of human colon cancer using 11701-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon cancer using 11701-1-AP

    IHC staining of human colon cancer using 11701-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 11701-1-AP

    IF Staining of HeLa using 11701-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11701-1-AP (TSFM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

TSFM Polyclonal antibody

TSFM Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11701-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

EF-TsMt, EF-Ts, EF Tsmt, EF TS, COXPD3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, PC-3 cells, HEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human kidney tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11701-1-AP targets TSFM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag2308

Product name: Recombinant human TSFM protein

Source: e coli.-derived, T-GST

Tag: GST

Domain: 1-346 aa of BC022862

Sequence: MSLLRSLRVFLVARTGSYPAGSLLRQSPQPRHTFYAGPRLSASASSKELLMKLRRKTGYSFVNCKKALETCGGDLKQAEIWLHKEAQKEGWSKAAKLQGRKTKEGLIGLLQEGNTTVLVEVNCETDFVSRNLKFQLLVQQVALGTMMHCQTLKDQPSAYSKVQWLTPVNLALWEAEAGGSLEGFLNSSELSGLPAGPDREGSLKDQLALAIGKLGENMILKRAAWVKVPSGFYVGSYVHGAMQSPSLHKLVLGKYGALVICETSEQKTNLEDVGRRLGQHVVGMAPLSVGSLDDEPGGEAETKMLSQPYLLDPSITLGQYVQPQGVSVVDFVRFECGEGEEAAETE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Ts translation elongation factor, mitochondrial
别名 EF-TsMt, EF-Ts, EF Tsmt, EF TS, COXPD3
计算分子量 35 kDa
观测分子量 35 kDa
GenBank蛋白编号BC022862
基因名称 TSFM
Gene ID (NCBI) 10102
RRIDAB_2207953
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43897
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TSFM antibody 11701-1-APDownload protocol
IHC protocol for TSFM antibody 11701-1-APDownload protocol
IF protocol for TSFM antibody 11701-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Biol Rep

Short-term regulation of TSFM level does not alter amyloidogenesis and mitochondrial function in type-specific cells

Authors - Xiao-Yun Li
  • KD Validated
View Article
humanWB

Cell Death Dis

piR-26441 inhibits mitochondrial oxidative phosphorylation and tumorigenesis in ovarian cancer through m6A modification by interacting with YTHDC1

Authors - Jing Yuan
View Article