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验证数据展示

  • WB analysis using 84102-6-RR

    WB analysis using 84102-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84102-6-RR (TSEN34 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 84102-6-RR

    IF Staining of MCF-7 using 84102-6-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TSEN34 antibody (84102-6-RR, Clone: 241155D2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

TSEN34 Recombinant antibody

TSEN34 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84102-6-RR

产品说明书

CloneNo. 241155D2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

SEN34, PCH2C, LENG5, HsSen34, EC:4.6.1.16

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, A431 cells, A549 cells, HeLa cells, MCF-7 cells, HCT 116 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84102-6-RR targets TSEN34 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 TSEN34 fusion protein Ag35120 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tRNA splicing endonuclease 34 homolog (S. cerevisiae)
别名 SEN34, PCH2C, LENG5, HsSen34, EC:4.6.1.16
计算分子量 34 kDa
观测分子量34 kDa
GenBank蛋白编号BC020805
基因名称 TSEN34
Gene ID (NCBI) 79042
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9BSV6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The human tRNA splicing endonuclease consists of a heterotetramer complex formed by the four TSEN proteins, which were identified by homology with their yeast counterparts. TSEN2 and TSEN34 are the catalytic subunits, which form a compound active site, whereas TSEN54 and TSEN15 are structural proteins. (PMID: 27392077)

实验方案

Product Specific Protocols
WB protocol for TSEN34 antibody 84102-6-RRDownload protocol
IF protocol for TSEN34 antibody 84102-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols