• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 31212-1-AP

    WB analysis using 31212-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31212-1-AP (TSEN34 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 31212-1-AP

    WB analysis of MCF-7 using 31212-1-AP

    WB result of TSEN34 antibody (31212-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TSEN34 transfected MCF-7 cells.

  • IF Staining of MCF-7 using 31212-1-AP

    IF Staining of MCF-7 using 31212-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TSEN34 antibody (31212-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Featured Product
  • KD/KO验证

TSEN34 Polyclonal antibody

TSEN34 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31212-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

EC:4.6.1.16, HsSen34, LENG5, PCH2C, SEN34

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHCT 116 cells, HEK-293 cells, MCF-7 cells, HeLa cells, HEK-293T cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31212-1-AP targets TSEN34 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 TSEN34 fusion protein Ag35120 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tRNA splicing endonuclease 34 homolog (S. cerevisiae)
别名 EC:4.6.1.16, HsSen34, LENG5, PCH2C, SEN34
计算分子量 34 kDa
观测分子量34-36 kDa
GenBank蛋白编号BC020805
基因名称 TSEN34
Gene ID (NCBI) 79042
RRIDAB_3669904
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9BSV6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The human tRNA splicing endonuclease consists of a heterotetramer complex formed by the four TSEN proteins, which were identified by homology with their yeast counterparts. TSEN2 and TSEN34 are the catalytic subunits, which form a compound active site, whereas TSEN54 and TSEN15 are structural proteins. (PMID: 27392077)

实验方案

Product Specific Protocols
WB protocol for TSEN34 antibody 31212-1-APDownload protocol
IF protocol for TSEN34 antibody 31212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols