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GILZ Polyclonal antibody

GILZ Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12352-1-AP

Print datasheet

Synonyms

DIP, DSIP immunoreactive peptide, DSIPI, GILZ, hDIP, Protein DIP, TSC 22 like protein, TSC 22 related protein, TSC 22R, TSC22 domain family protein 3, TSC22 domain family, member 3, TSC22D3

验证数据展示

  • WB analysis of mouse brain using 12352-1-AP

    WB analysis of mouse brain using 12352-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12352-1-AP (GILZ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 12352-1-AP

    IF Staining of U2OS using 12352-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using GILZ antibody (12352-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IF detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy TSC22D3/GILZ Ready-To-Use IHC Kit. TSC22D3/GILZ primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

12352-1-AP targets GILZ in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Immunogen GILZ fusion protein Ag3015 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TSC22 domain family, member 3
Synonyms DIP, DSIP immunoreactive peptide, DSIPI, GILZ, hDIP, Protein DIP, TSC 22 like protein, TSC 22 related protein, TSC 22R, TSC22 domain family protein 3, TSC22 domain family, member 3, TSC22D3
Calculated Molecular Weight 134 aa, 15 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC018148
Gene Symbol TSC22D3
Gene ID (NCBI) 1831
RRIDAB_2272196
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99576
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GILZ antibody 12352-1-APDownload protocol
IF protocol for GILZ antibody 12352-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Invest Ophthalmol Vis Sci

Glucocorticoid-Induced Leucine Zipper Protects the Retina From Light-Induced Retinal Degeneration by Inducing Bcl-xL in Rats.

Authors - Ruiping Gu
View Article
ratWB

Invest Ophthalmol Vis Sci

Glucocorticoid-Induced Leucine Zipper Suppresses ICAM-1 and MCP-1 Expression by Dephosphorylation of NF-κB p65 in Retinal Endothelial Cells.

Authors - Ruiping Gu
View Article
humanWB

Exp Eye Res

Glucocorticoid-induced leucine zipper overexpression inhibits lipopolysaccharide-induced retinal inflammation in rats.

Authors - Ruiping Gu
View Article