验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, MDA-MB-453s cells, HepG2 cells, SH-SY5Y cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human kidney tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
25418-1-AP targets TSC22D2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | TSC22D2 fusion protein Ag22294 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TSC22 domain family, member 2 |
| 别名 | TSC22 domain family protein 2, TILZ4c, TILZ4b, TILZ4a, TILZ4 |
| 计算分子量 | 780 aa, 79 kDa |
| 观测分子量 | 100-110 kDa |
| GenBank蛋白编号 | BC044643 |
| 基因名称 | TSC22D2 |
| Gene ID (NCBI) | 9819 |
| RRID | AB_2880069 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75157 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TSC22D2 (transforming growth factor β-stimulated clone 22 domain family, member 2) is a member of the TSC-22 domain family comprising putative transcription factors that are characterized by a carboxy-terminal leucine zipper and an adjacent TSC-box. TSC22D2 is expressed at low levels in CRC and that TSC22D2 overexpression significantly inhibited the growth rate of cancer cells (PMID: 27573352).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TSC22D2 antibody 25418-1-AP | Download protocol |
| IHC protocol for TSC22D2 antibody 25418-1-AP | Download protocol |
| IF protocol for TSC22D2 antibody 25418-1-AP | Download protocol |
| IP protocol for TSC22D2 antibody 25418-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
