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TSC22D1 Polyclonal antibody

TSC22D1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10214-1-Ig

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

RP11 269C23.2, Regulatory protein TSC-22, Regulatory protein TSC 22, KIAA1994, HUCEP-2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inhuman brain tissue, A549 cells, rat brain tissue
Positive IP detected inrat brain tissue
Positive IHC detected inhuman brain tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy TSC22D1 Ready-To-Use IHC Kit. TSC22D1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10214-1-Ig targets TSC22D1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, rat
文献引用反应性human, mouse
免疫原 TSC22D1 fusion protein Ag0287 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TSC22 domain family, member 1
别名 RP11 269C23.2, Regulatory protein TSC-22, Regulatory protein TSC 22, KIAA1994, HUCEP-2
计算分子量 16 kDa
观测分子量 16 kDa
GenBank蛋白编号BC000456
基因名称 TSC22D1
Gene ID (NCBI) 8848
RRIDAB_2272193
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15714
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSC22 domain family 1, transforming growth factor beta 1 induced transcript 4, (TSC22D1, synonyms: TSC22, TGFB1I4, MGC17597) is a member of a family of leucine zipper containing transcription factors with repressor activity and belongs to the large family of early response genes. TSC-22 regulates cell growth and differentiation, and cell death. Leucine zipper structure of TSC22 inhibits the anchorage independent growth of cancer cells. TSC-22 is also an important downstream component of peroxisome proliferator-activated receptor (PPAR) and TGF-beta signaling during intestinal epithelial cell differentiation. This antibody is a rabbit polyclonal antibody raised against an internal region of human TSC22D1.

实验方案

Product Specific Protocols
WB protocol for TSC22D1 antibody 10214-1-IgDownload protocol
IHC protocol for TSC22D1 antibody 10214-1-IgDownload protocol
IF protocol for TSC22D1 antibody 10214-1-IgDownload protocol
IP protocol for TSC22D1 antibody 10214-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Death Differ

TSC-22D1 isoforms have opposing roles in mammary epithelial cell survival.

Authors - Huser C A CA
human,mouseWB,IHC

Clin Cancer Res

Transforming Growth Factor-{beta}-Stimulated Clone-22 Is an Androgen-Regulated Gene That Enhances Apoptosis in Prostate Cancer following Insulin-Like Growth Factor-I Receptor Inhibition.

Authors - Sprenger Cynthia C T CC
  • KD Validated
humanWB,IF

Int J Mol Sci

Identification of Binding Proteins for TSC22D1 Family Proteins Using Mass Spectrometry.

Authors - Ryouta Kamimura
mouseIP,WB

FEBS J

Multiple TSC22D4 iso-/phospho-glycoforms display idiosyncratic subcellular localizations and interacting protein partners.

Authors - Canterini Sonia S
humanWB,IF

Am J Physiol Heart Circ Physiol

Transforming growth factor-beta1 regulation of C-type natriuretic peptide expression in human vascular smooth muscle cells: dependence on TSC22D1.

Authors - Mendon?a Maria C MC
humanWB,IHC

Biochem Biophys Res Commun

Low levels of TSC22 enhance tumorigenesis by inducing cell proliferation in colorectal cancer.

Authors - Yong Zhou
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