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验证数据展示

  • WB analysis using 27892-1-AP (same clone as 27892-1-PBS)

    WB analysis using 27892-1-AP (same clone as 27892-1-PBS)

    SH-SY5Y cell lysates (heated at 100℃, heated at 37℃) were subjected to SDS PAGE followed by western blot with 27892-1-AP (TRPV4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27892-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 27892-1-AP (same clone as 27892-1-PBS)

    IF Staining of SH-SY5Y using 27892-1-AP (same clone as 27892-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using TRPV4 antibody (27892-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 27892-1-PBS in a different storage buffer formulation.

TRPV4 Polyclonal antibody, PBS Only

TRPV4 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 27892-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

Osm 9 like TRP channel 4, Osm-9-like TRP channel 4, OTRPC4, TRP12, Vanilloid receptor-like channel 2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

27892-1-PBS targets TRPV4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag27253

Product name: Recombinant human TRPV4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-210 aa of NM_001177428.1

Sequence: MADSSEGPRAGPGEVAELPGDESGTPGGEAFPLSSLANLFEGEDGSLSPSPADASRPAGPGDGRPNLRMKFQGAFRKGVPNPIDLLESTLYESSVVPGPKKAPMDSLFDYGTYRHHSSDNKRWRKKIIEKQPQSPKAPAPQPPPILKVFNRPILFDIVSRGSTADLDGLLPFLLTHKKRLTDEEFREPSTGKTCLPKALLNLSNGRNDTI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transient receptor potential cation channel, subfamily V, member 4
别名 Osm 9 like TRP channel 4, Osm-9-like TRP channel 4, OTRPC4, TRP12, Vanilloid receptor-like channel 2
计算分子量 98 kDa
观测分子量 85~98 kDa
GenBank蛋白编号NM_001177428.1
基因名称 TRPV4
Gene ID (NCBI) 59341
RRIDAB_3669629
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HBA0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRPV4, also named as VRL2 and VROAC, belongs to the transient receptor family and TrpV subfamily. It is a non-selective calcium permeant cation channel protein which involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. It is activated by low pH, citrate and phorbol esters. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Defects in TRPV4 are the cause of brachyolmia type 3 (BRAC3), spondylometaphyseal dysplasia Kozlowski type (SMDK) and metatropic dysplasia (MTD). Multiple transcript variants encoding different isoforms have been found.

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