验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, MDA-MB-468 cells, T-47D cells, HEK-293 cells, Y79 cells, HUVEC cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 37 publications below |
| CoIP | See 1 publications below |
产品信息
66983-1-Ig targets TRPV1 in WB, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, sheep |
| 免疫原 |
CatNo: Ag18683 Product name: Recombinant human TRPV1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-203 aa of BC132820 Sequence: MKKWSSTDLGAAADPLQKDTCPDPLDGDPNSRPPPAKPQLSTAKSRTRLFGKGDSEEAFPVDCPHEEGELDSCPTITVSPVITIQRPGDGPTGARLLSQDSVAASTEKTLRLYDRRSIFEAVAQNNCQDLESLLLFLQKSKKHLTDNEFKDPETGKTCLLKAMLNLHDGQNTTIPLLLEIARQTDSLKELVNASYTDSYYKGQ 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | transient receptor potential cation channel, subfamily V, member 1 |
| 别名 | 1A3C9, Osm 9 like TRP channel 1, Osm-9-like TRP channel 1, OTRPC1, Vanilloid receptor 1 |
| 计算分子量 | 839 aa, 95 kDa |
| 观测分子量 | 105 kDa |
| GenBank蛋白编号 | BC132820 |
| 基因名称 | TRPV1 |
| Gene ID (NCBI) | 7442 |
| RRID | AB_2882302 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q8NER1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRPV1, also named as VR1 or OTRPC1, belongs to the transient receptor potential (TRP) family and TRPV subfamily (PMID: 17579562). TRPV1 is a ligand-activated non-selective calcium permeant cation channel expressed predominantly by sensory neurons (PMID: 10821274). It is involved in detection of noxious chemical and thermal stimuli. TRPV1 plays an important role in inflammatory thermal hyperalgesia (PMID: 10764638; 10821274).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRPV1 antibody 66983-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Sleep deficiency exacerbates periodontal inflammation via trigeminal TRPV1 neurons | ||
Phytomedicine Yu-Xue-Bi capsule ameliorates aggressive synovitis and joint damage in rheumatoid arthritis via modulating the SUCNR1/HIF-1α/TRPV1 axis | ||
Int J Mol Sci The Contribution of TSLP Activation to Hyperalgesia in Dorsal Root Ganglia Neurons of a Rat. | ||
Biochim Biophys Acta Mol Basis Dis Palmitic acid and trans-4-hydroxy-3-methoxycinnamate, the active ingredients of Yaobishu formula, reduce inflammation and pain by regulating gut microbiota and metabolic changes after lumbar disc herniation to activate autophagy and the Wnt/β-catenin pathway | ||
EBioMedicine Single cell RNA-seq analysis identifies ferroptotic chondrocyte cluster and reveals TRPV1 as an anti-ferroptotic target in osteoarthritis | ||
Int J Mol Sci Luteolin Alleviates Oxidative Stress in Chronic Obstructive Pulmonary Disease Induced by Cigarette Smoke via Modulation of the TRPV1 and CYP2A13/NRF2 Signaling Pathways |
