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验证数据展示

  • IHC staining of human liver cancer using 15541-1-AP

    IHC staining of human liver cancer using 15541-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15541-1-AP (TRPT1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 15541-1-AP

    IHC staining of human liver cancer using 15541-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15541-1-AP (TRPT1 Antibody) at dilution of 1:50 (under 40x lens).

TRPT1 Polyclonal antibody

TRPT1 Polyclonal Antibody for IHC, ELISA
Cat No. 15541-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

IHC, ELISA and More (1)

别名

tRNA 2 phosphotransferase 1, tRNA phosphotransferase 1, TRPT1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15541-1-AP targets TRPT1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性rat
免疫原

CatNo: Ag7897

Product name: Recombinant human TRPT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-204 aa of BC005133

Sequence: MGADGFVPLGTLLQLPQFRGFSAEDVQRVVDTNRKQRFALQLGDPSTGLLIRANQGHSLQVPKLELMPLETPQALPPMLVHGTFWKHWPSILLKGLSCQGRTHIHLAPGLPGDPGIISGMRSHCEIAVFIDGPLALADGIPFFRSANGVILTPGNTDGFLLPKYFKEALQLRPTRKPLSLAGDEETECQSSPKHSSRERRRIQQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tRNA phosphotransferase 1
别名 tRNA 2 phosphotransferase 1, tRNA phosphotransferase 1, TRPT1
计算分子量 28 kDa
GenBank蛋白编号BC005133
基因名称 TRPT1
Gene ID (NCBI) 83707
RRIDAB_2878149
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86TN4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRPT1 catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate. It has four isoforms with MW 22-24 and 27-29 kDa.

实验方案

Product Specific Protocols
IHC protocol for TRPT1 antibody 15541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Int J Mol Sci

Impaired 26S Proteasome Assembly Precedes Neuronal Loss in Mutant UBQLN2 Rats.

Authors - Wenjuan Zhang
View Article