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验证数据展示

  • WB analysis using 28753-1-AP

    WB analysis using 28753-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28753-1-AP (TRPS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 28753-1-AP

    IHC staining of human ovary cancer using 28753-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28753-1-AP (TRPS1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 28753-1-AP

    IHC staining of human ovary cancer using 28753-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28753-1-AP (TRPS1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • KD/KO验证

TRPS1 Polyclonal antibody

TRPS1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28753-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Tricho-rhino-phalangeal syndrome type I protein, LGCR, GC79

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28753-1-AP targets TRPS1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30188

Product name: Recombinant human TRPS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1158-1281 aa of NM_014112

Sequence: SDNDIPLDLAIKHSRPGPTANGASKEKTKAPPNVKNEGPLNVVKTEKVDRSTQDELSTKCVHCGIVFLDEVMYALHMSCHGDSGPFQCSICQHLCTDKYDFTTHIQRGLHRNNAQVEKNGKPKE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 trichorhinophalangeal syndrome I
别名 Tricho-rhino-phalangeal syndrome type I protein, LGCR, GC79
计算分子量 142 kDa
观测分子量142 kDa
GenBank蛋白编号NM_014112
基因名称 TRPS1
Gene ID (NCBI) 7227
RRIDAB_2881209
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UHF7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRPS1 is a zinc finger transcriptional repressor involved in the regulation of chondrocyte and perichondrium development, containing a GATA-type zinc finger through which it binds to DNA. with nine zinc-finger domains and two C-terminal Ikaros-like zinc fingers. Its reperssible function was dependent on the integrity of the Trps1 GATA-type zinc-finger domain and also required the C-terminal 119 amino acids of the protein, which harbor the two Ikaros-like zinc-finger domains

实验方案

Product Specific Protocols
IHC protocol for TRPS1 antibody 28753-1-APDownload protocol
WB protocol for TRPS1 antibody 28753-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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