验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse kidney tissue, rat brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human prostate cancer tissue, human kidney tissue, mouse brain tissue, mouse kidney tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20003-1-AP targets TRPS1 in WB, IHC, IF/ICC, IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IF, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, chIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | trichorhinophalangeal syndrome I |
| 别名 | GC79, LGCR, TRPS1, Zinc finger protein GC79 |
| 计算分子量 | 142 kDa |
| 观测分子量 | 135-142 kDa |
| GenBank蛋白编号 | NM_014112 |
| 基因名称 | TRPS1 |
| Gene ID (NCBI) | 7227 |
| RRID | AB_10666999 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UHF7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRPS1, also named as GC79 and LGCR, is a transcriptional repressor. TRPS1 may act to restrict expression of GATA-regulated genes at selected sites and stages in vertebrate development. It might be involved in prostate cancer apoptosis. Defects in TRPS1 are the cause of tricho-rhino-phalangeal syndrome type 1/2/3 (TRPS1) (TRPS2) (TRPS3). The antibody recognizes the C-term of TRPS1.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for TRPS1 antibody 20003-1-AP | Download protocol |
| IHC protocol for TRPS1 antibody 20003-1-AP | Download protocol |
| WB protocol for TRPS1 antibody 20003-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Multi-omic analysis reveals divergent molecular events in scarring and regenerative wound healing. | ||
J Pathol Down-regulation of TRPS1 stimulates epithelial-mesenchymal transition and metastasis through repression of FOXA1.
| ||
Int J Mol Med Pro‑differentiating compounds for human intervertebral disc cells are present in Violina pumpkin leaf extracts | ||
J Biol Chem Dual Role of the Trps1 Transcription Factor in Dentin Mineralization.
| ||
J Cell Physiol Expression and function of the P2X7 receptor in human osteoblasts: The role of NFATc1 transcription factor. | ||
Life Sci Decellularized extracellular matrix-based scaffold and hypoxic priming: A promising combination to improve the phenotype of degenerate intervertebral disc cells. |
