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TRPM7 Polyclonal antibody

TRPM7 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 55251-1-AP

Print datasheet

Synonyms

CHAK, CHAK1, Channel kinase 1, LTrpC 7, LTRPC7, TRP PLIK, TRPM7

验证数据展示

  • WB analysis using 55251-1-AP

    WB analysis using 55251-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55251-1-AP (TRPM7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55251-1-AP targets TRPM7 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transient receptor potential cation channel, subfamily M, member 7
Synonyms CHAK, CHAK1, Channel kinase 1, LTrpC 7, LTRPC7, TRP PLIK, TRPM7
Calculated Molecular Weight 213 kDa
Observed Molecular Weight220 kDa
GenBank Accession NumberNM_017672
Gene Symbol TRPM7
Gene ID (NCBI) 54822
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96QT4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transient receptor potential melastatin 7 (TRPM7), along with the closely related TRPM6, are unique channels that have dual operations: cation permeability and kinase activity. In contrast to the limited tissue distribution of TRPM6, TRPM7 is widely expressed among tissues and is therefore implicated in a variety of cellular functions physiologically and pathophysiologically. The discovery of TRPM7's unique structure imparting dual ion channel and kinase activities shed light onto novel and peculiar biological functions, such as Mg2+ homeostasis, cellular Ca2+ flickering, and even intranuclear transcriptional regulation by a cleaved kinase domain translocated to nuclei. The calculated MW of TRPM7 is 213 kDa, 55251-1-AP can detect a band around 220 kDa. (PMID: 32503408)

实验方案

Product Specific Protocols
WB protocol for TRPM7 antibody 55251-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols