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验证数据展示

  • FC experiment of LNCaP using 98691-2-RR

    FC experiment of LNCaP using 98691-2-RR

    1x10^6 LNCaP cells were intracellularly stained with 0.25 ug Anti-Human TRPM5 Rabbit RecAb (98691-2-RR, Clone: 251123E12) (red) or 0.25 ug Isotype Control (blue), and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • IHC staining of human pancreas using 98691-2-RR

    IHC staining of human pancreas using 98691-2-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 98691-2-RR (TRPM5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas using 98691-2-RR

    IHC staining of human pancreas using 98691-2-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 98691-2-RR (TRPM5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Anti-Human TRPM5 Rabbit Recombinant Antibody

TRPM5 Uni-rAb® Recombinant Antibody for IHC, FC (Intra)
Cat No. 98691-2-RR

产品说明书

CloneNo. 251123E12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, FC (Intra)

别名

LTrpC 5, LTrpC5, LTrpC-5, MLSN1- and TRP-related gene 1 protein, MTR1

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inLNCaP cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98691-2-RR targets TRPM5 in IHC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IHC, FC (Intra) Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transient receptor potential cation channel, subfamily M, member 5
别名 LTrpC 5, LTrpC5, LTrpC-5, MLSN1- and TRP-related gene 1 protein, MTR1
计算分子量98 kDa, 131 kDa
GenBank蛋白编号BC093787
基因名称 TRPM5
Gene ID (NCBI) 29850
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NZQ8
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Transient receptor potential (TRP) proteins are a diverse family of proteins with structural features typical of ion channels (PMID: 14634208). TRPM5 is a member of the TRPM (melastatin-like) subfamily which are Ca(2+)-permeable cation channels localized predominantly to the plasma membrane (PMID: 11864597). TRPM5 plays a central role in taste transduction (PMID: 17610722). TRPM5 is implicated in enhancing TRPA1 expression and may be involved in regulating insulin secretion (PMID: 21932052).

实验方案

Product Specific Protocols
FC protocol for TRPM5 antibody 98691-2-RRDownload protocol
IHC protocol for TRPM5 antibody 98691-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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