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TRPM3 Polyclonal antibody

TRPM3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 12724-1-AP

Print datasheet

Synonyms

GON 2, LTRPC3, MLSN2, TRPM3

验证数据展示

  • WB analysis using 12724-1-AP

    WB analysis using 12724-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12724-1-AP (TRPM3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12724-1-AP targets TRPM3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen TRPM3 fusion protein Ag3421 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transient receptor potential cation channel, subfamily M, member 3
Synonyms GON 2, LTRPC3, MLSN2, TRPM3
Calculated Molecular Weight 1732 aa, 197 kDa
Observed Molecular Weight160-210 kDa
GenBank Accession NumberBC022454
Gene Symbol TRPM3
Gene ID (NCBI) 80036
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HCF6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The transient receptor potential melastatin-3 (TRPM3) channel belongs to the family of transient receptor potential (TRP) cation channels that are expressed in a variety of tissues and cell types, including kidney, brain, dorsal root ganglia, cardiomyocytes and pancreatic beta-cells. TRPM3 channels can be activated by the neurosteroid pregnenolone sulfate, and they are regulated by phosphoinositides, and perhaps by calmodulin. Stimulation of TRPM3 channels is connected with heat sensation by somatosensory neurons, insulin secretion by pancreatic beta-cells, regulation of neurotransmitter release, iris constriction, and tumor promotion. The calculated MW of TRPM3 is 197 kDa, 12724-1-AP can detect a band around 160 kDa, which likely corresponds to different splice variant. (PMID: 28720517,21198543)

实验方案

Product Specific Protocols
WB protocol for TRPM3 antibody 12724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols