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验证数据展示

  • IF Staining of SH-SY5Y using 55111-1-AP

    IF Staining of SH-SY5Y using 55111-1-AP

    Immunofluorescent analysis of SH-SY5Y cells using 55111-1-AP (TRPM1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • IHC staining of human lung cancer using 55111-1-AP

    IHC staining of human lung cancer using 55111-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55111-1-AP (TRPM1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 55111-1-AP

    IHC staining of human lung cancer using 55111-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55111-1-AP (TRPM1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of SH-SY5Y using 55111-1-AP

    FC experiment of SH-SY5Y using 55111-1-AP

    1X10^6 SH-SY5Y cells were stained with 0.2ug TRPM1 antibody (55111-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • IHC staining of human malignant melanoma using 55111-1-AP

    IHC staining of human malignant melanoma using 55111-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 55111-1-AP (TRPM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human malignant melanoma using 55111-1-AP

    IHC staining of human malignant melanoma using 55111-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 55111-1-AP (TRPM1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TRPM1 Polyclonal antibody

TRPM1 Polyclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 55111-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

LTRPC1, Melastatin 1, MLSN, MLSN1, TRPM1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells, A431 cells
Positive FC (Intra) detected inSH-SY5Y cells
Positive FC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:25-1:100
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55111-1-AP targets TRPM1 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transient receptor potential cation channel, subfamily M, member 1
别名 LTRPC1, Melastatin 1, MLSN, MLSN1, TRPM1
计算分子量 182 kDa
GenBank蛋白编号NM_002420
基因名称 TRPM1
Gene ID (NCBI) 4308
RRIDAB_2881269
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z4N2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for TRPM1 antibody 55111-1-APDownload protocol
IF protocol for TRPM1 antibody 55111-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols