验证数据展示
经过测试的应用
| Positive WB detected in | mouse cerebellum tissue, HEK-293 cells, 37℃ incubated mouse brain tissue, unboiled rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84019-5-RR targets TRPC3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | TRPC3 fusion protein Ag13024 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | transient receptor potential cation channel, subfamily C, member 3 |
| 别名 | Transient receptor protein 3, Short transient receptor potential channel 3, hTrp-3, hTrp3, hTrp 3 |
| 计算分子量 | 848 aa, 97 kDa |
| 观测分子量 | 105 kDa, 97 kDa |
| GenBank蛋白编号 | BC093682 |
| 基因名称 | TRPC3 |
| Gene ID (NCBI) | 7222 |
| RRID | AB_3671588 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q13507 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRPC3 (Short transient receptor potential channel 3), also known as TRP3. It is expected to be located in the cell membrane, which is expressed predominantly in brain and at much lower levels in ovary, colon, small intestine, lung, prostate, placenta and testis. TRPC3 is one of the classical members of the mammalian transient receptor potential (TRP) superfamily of ion channels. TRPC3 is a molecule with intriguing sensory features including the direct recognition of and activation by diacylglycerols (DAG). Although TRPC3 channels are ubiquitously expressed, they appear to control functions of the cardiovascular system and the brain in a highly specific manner. Moreover, a role of TRPC3 in immunity, cancer, and tissue remodeling has been proposed, generating much interest in TRPC3 as a target for pharmacological intervention. The molecular weight of TRPC3 is 105 kDa, and it can recognize another isoform. (PMID: 30037143)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRPC3 antibody 84019-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |