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TRPC1 Polyclonal antibody

TRPC1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse , rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 24727-1-AP

Print datasheet

Synonyms

HTRP 1, Transient receptor protein 1, TRP 1, TRP1, TRPC1

验证数据展示

  • WB analysis using 24727-1-AP

    WB analysis using 24727-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24727-1-AP (TRPC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 24727-1-AP

    IHC staining of rat brain using 24727-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24727-1-AP (TRPC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 24727-1-AP

    IHC staining of rat brain using 24727-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24727-1-AP (TRPC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24727-1-AP targets TRPC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse , rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse , rat
Immunogen TRPC1 fusion protein Ag16488 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transient receptor potential cation channel, subfamily C, member 1
Synonyms HTRP 1, Transient receptor protein 1, TRP 1, TRP1, TRPC1
Calculated Molecular Weight 793 aa, 91 kDa
Observed Molecular Weight120 kDa
GenBank Accession NumberBC113953
Gene Symbol TRPC1
Gene ID (NCBI) 7220
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48995
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRPC1, also named as TRP1, belongs to the transient receptor family and STrpC subfamily. TRPC1 is thought to form a receptor-activated non-selective calcium permeant cation channel. It has been reported that the mGluR1 evoked slow EPSC is mediated by the TRPC1 cation channel. It is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors.

实验方案

Product Specific Protocols
WB protocol for TRPC1 antibody 24727-1-APDownload protocol
IHC protocol for TRPC1 antibody 24727-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols