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验证数据展示

  • WB analysis using 25226-1-AP

    WB analysis using 25226-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25226-1-AP (TRPA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

TRPA1 Polyclonal antibody

TRPA1 Polyclonal Antibody for WB, ELISA
Cat No. 25226-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Wasabi receptor, p120, ANKTM1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, U2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25226-1-AP targets TRPA1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 TRPA1 fusion protein Ag18685 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transient receptor potential cation channel, subfamily A, member 1
别名 Wasabi receptor, p120, ANKTM1
计算分子量 1119 aa, 128 kDa
观测分子量130 kDa
GenBank蛋白编号BC153003
基因名称 TRPA1
Gene ID (NCBI) 8989
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75762
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRPA1 (Transient Receptor Potential Ankyrin 1) is a member of the transient receptor potential (TRP) channel family and is a non-selective cation channel. TRPA1 is predominantly expressed in sensory neurons and is involved in a variety of physiological activities, such as stabilizing cellular membrane potentials, maintaining cellular fluid homeostasis, and regulating intercellular signaling. TRPA1 is a multimodal cell membrane receptor that senses different stimuli and upon activation generates action potential signals via osmolality, temperature and inflammatory factors. TRPA1 has been associated with neurogenic inflammation, osteoarthritis, atopic dermatitis, asthma, inflammatory bowel disease, migraine headache, cancer pain, gout and other diseases, and it is a promising target for drug therapy.

实验方案

Product Specific Protocols
WB protocol for TRPA1 antibody 25226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols