验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, rat skin tissue, A431 cells, HL-60 cells, MCF-7 cells, COS-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
88100-1-RR targets TRP2/DCT in WB, ELISA applications and shows reactivity with human, rat, monkey samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, rat, monkey |
| 免疫原 |
CatNo: Ag26165 Product name: Recombinant human DCT protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-350 aa of BC028311 Sequence: MSPLWWGFLLSCLGCKILPGAQGQFPRVCMTVDSLVNKECCPRLGAESANVCGSQQGRGQCTEVRADTRPWSGPYILRNQDDRELWPRKFFHRTCKCTGNFAGYNCGDCKFGWTGPNCERKKPPVIRQNIHSLSPQEREQFLGALDLAKKRVHPDYVITTQHWLGLLGPNGTQPQFANCSVYDFFVWLHYYSVRDTLLGPGRPYRAIDFSHQGPAFVTWHRYHLLCLERDLQRLIGNESFALPYWNFATGRNECDVCTDQLFGAARPDDPTLISRNSRFSSWETVCDSLDDYNHLVTLCNGTYEGLLRRNQMGRNSMKLPTLKDIRDCLSLQKFDNPPFFQNSTFSFRNA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2) |
| 别名 | DCT, DT, EC:5.3.3.12, L-dopachrome Delta-isomerase, L-dopachrome tautomerase |
| 计算分子量 | 519 aa, 59 kDa |
| 观测分子量 | 59-63 kDa |
| GenBank蛋白编号 | BC028311 |
| 基因名称 | DCT |
| Gene ID (NCBI) | 1638 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P40126 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DCT (L-dopachrome Delta-isomerase) is also named as TYRP2,TRP2 and belongs to the tyrosinase family. Human TYRP2 protein has 83% identity and 90% similarity to the mouse sequence and has all the structural characteristics of the tyrosinase protein family, including a signal peptide, 15 conserved cysteine residues, 2 copper-binding domains, and a C-terminal membrane-spanning region (PMID:8206391). TYRP2 is involved in neural progenitor cell proliferation and has been implicated in drug and radiation resistance in malignant melanoma. Mutations in DCT cause oculocutaneous albinism type VIII (OCA8), and TYRP2 serves as both an early melanoblast marker and a melanoma-associated antigen with clinical relevance as a potential biomarker in immune checkpoint therapy.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRP2/DCT antibody 88100-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
