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验证数据展示

  • WB analysis of MCF-7 using 12718-1-AP

    WB analysis of MCF-7 using 12718-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12718-1-AP (TROVE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 12718-1-AP

    IF Staining of PC-3 using 12718-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using TROVE2 antibody (12718-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

TROVE2 Polyclonal antibody

TROVE2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 12718-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

SS A, RoRNP, Ro60 autoantigen, RO60, RNA-binding protein RO60

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF7 cells, HeLa cells, mouse thymus tissue, PC-3 cells, rat spleen tissue
Positive IF/ICC detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12718-1-AP targets TROVE2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 TROVE2 fusion protein Ag3412 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TROVE domain family, member 2
别名 SS A, RoRNP, Ro60 autoantigen, RO60, RNA-binding protein RO60
计算分子量 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC036658
基因名称 TROVE2
Gene ID (NCBI) 6738
RRIDAB_2209756
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10155
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TROVE2, also named as RO60 or RoRNP, is a 538 amino acid protein, which is localized in cytoplasmic mRNP granules containing untranslated mRNAs. TROVE2 as a RNA-binding protein that binds to misfolded non-coding RNAs, pre-5S rRNA, and several small cytoplasmic RNA molecules known as Y RNAs. TROVE2 may stabilize some of these RNAs and protect them from degradation (PubMed:18056422). TROVE2 binds to endogenous Alu retroelements which are induced by type I IFN and stimulate porinflammaotry cytokine secretion.

实验方案

Product Specific Protocols
WB protocol for TROVE2 antibody 12718-1-APDownload protocol
IF protocol for TROVE2 antibody 12718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

Selective sorting and secretion of hY4 RNA fragments into extracellular vesicles mediated by methylated YBX1 to promote lung cancer progression.

Authors - Chuang Li
View Article