验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HCT 116 cells, PC-3 cells, HT-29 cells, Jurkat cells |
| Positive IHC detected in | human gliomas tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy TROAP Ready-To-Use IHC Kit. TROAP primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 4 publications below |
| WB | See 8 publications below |
| IHC | See 5 publications below |
产品信息
13634-1-AP targets TROAP in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | TROAP fusion protein Ag4562 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | trophinin associated protein (tastin) |
| 别名 | TASTIN, TROAP, Trophinin assisting protein, Trophinin associated protein |
| 计算分子量 | 778 aa, 84 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | BC032583 |
| 基因名称 | TROAP |
| Gene ID (NCBI) | 10024 |
| RRID | AB_10597857 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q12815 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TROAP, also known as tastin, was first cloned from human epithelial cells in 1995. It as a cytoplasmic protein is composed of 778 amino acid residues and contains potential phosphorylation sites for protein kinases. TROAP highly expresses in bone marrow, testis and thymus, and is involved in centrosome integrity and spindle assembly during mitosis (PMID: 33500384). TROAP has been shown to promote some tumor progressions, such as those of breast and colorectal cancers (PMID: 33692939).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TROAP antibody 13634-1-AP | Download protocol |
| IHC protocol for TROAP antibody 13634-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Dev Cell The HCMV Assembly Compartment Is a Dynamic Golgi-Derived MTOC that Controls Nuclear Rotation and Virus Spread. | ||
Brief Bioinform Comprehensive assessment of cellular senescence in the tumor microenvironment. | ||
Biomed Pharmacother MicroRNA-519d-3p inhibits cell proliferation and migration by targeting TROAP in colorectal cancer.
| ||
Cell Cycle MiR-532-3p suppresses cell viability, migration and invasion of clear cell renal cell carcinoma through targeting TROAP. | ||
Front Oncol Downregulation of Methyltransferase-Like 14 Promotes Ovarian Cancer Cell Proliferation Through Stabilizing TROAP mRNA. | ||
CNS Neurosci Ther TROAP regulates cell cycle and promotes tumor progression through Wnt/β-Catenin signaling pathway in glioma cells. |
