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验证数据展示

  • IF Staining of HepG2 using 25376-1-AP

    IF Staining of HepG2 using 25376-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 25376-1-AP (TRMU antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse skeletal muscle using 25376-1-AP

    IHC staining of mouse skeletal muscle using 25376-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25376-1-AP (TRMU antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TRMU Polyclonal antibody

TRMU Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 25376-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

EC:2.8.1.14, Mitochondrial tRNA-specific 2-thiouridylase 1, MTO2, MTO2 homolog, MTU1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25376-1-AP targets TRMU in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag21930

Product name: Recombinant human TRMU protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-267 aa of BC027991

Sequence: MKKSLSRSTLRSPKGFSEIGLKLEMVSQDALRRTIFPLGGLTKEFVKKIAAENRLHHVLQKKESMGMCFIGKRNFEHFLLQYLQPRPGHFISIEDNKVLGTHKGWFLYTLGQRANIGGLREPWYVVEKDSVKGDVFVAPRTDHPALYRDLLRTSRVHWIAEEPPAALVRDKMMECHFRFRHQMALVPCVLTLNQDGTVWVTAVQAVRALATGQFAVFYKGDECLGSGKILRLGPSAYTLQKGQRRAGMATESPSDSPEDGPGLSPLL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase
别名 EC:2.8.1.14, Mitochondrial tRNA-specific 2-thiouridylase 1, MTO2, MTO2 homolog, MTU1
计算分子量 421 aa, 48 kDa
GenBank蛋白编号BC027991
基因名称 TRNT1
Gene ID (NCBI) 55687
RRIDAB_3085793
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75648
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for TRMU antibody 25376-1-APDownload protocol
IHC protocol for TRMU antibody 25376-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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