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TRNT1 Polyclonal antibody

TRNT1 Polyclonal Antibody for IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, ELISA

Conjugate

Unconjugated

Cat No : 25376-1-AP

Print datasheet

Synonyms

MTO2, MTO2 homolog, MTU1, TRMT, TRMT1, TRMU, TRNT1

验证数据展示

  • IF Staining of HepG2 using 25376-1-AP

    IF Staining of HepG2 using 25376-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 25376-1-AP (TRMU antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse skeletal muscle using 25376-1-AP

    IHC staining of mouse skeletal muscle using 25376-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25376-1-AP (TRMU antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25376-1-AP targets TRNT1 in IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, ELISA
Tested Reactivity human, mouse
Immunogen TRNT1 fusion protein Ag21930 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase
Synonyms MTO2, MTO2 homolog, MTU1, TRMT, TRMT1, TRMU, TRNT1
Calculated Molecular Weight 421 aa, 48 kDa
GenBank Accession NumberBC027991
Gene Symbol TRMU
Gene ID (NCBI) 55687
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75648
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for TRNT1 antibody 25376-1-APDownload protocol
IF protocol for TRNT1 antibody 25376-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols