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验证数据展示

  • WB analysis using 31169-1-AP

    WB analysis using 31169-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31169-1-AP (TRMT61A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of RAW 264.7 using 31169-1-AP

    WB analysis of RAW 264.7 using 31169-1-AP

    WB result of TRMT61A antibody (31169-1-AP; 1:3000; room temperature for 1.5 hours) with wild-type and TRMT61A knockout RAW 264.7 cells.

  • Featured Product
  • KD/KO验证

TRMT61A Polyclonal antibody

TRMT61A Polyclonal Antibody for WB, ELISA
Cat No. 31169-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

hTRM61, Gcd14p, GCD14, EC:2.1.1.220, EC:2.1.1.-

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected in5637 cells, RAW 264.7 cells, MCF-7 cells, U-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31169-1-AP targets TRMT61A in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 TRMT61A fusion protein Ag34396 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tRNA methyltransferase 61 homolog A (S. cerevisiae)
别名 hTRM61, Gcd14p, GCD14, EC:2.1.1.220, EC:2.1.1.-
观测分子量30-35 kDa
GenBank蛋白编号BC010167
基因名称 TRMT61A
Gene ID (NCBI) 115708
RRIDAB_3669880
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ96FX7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

N1-methyladenosine (m1A) is an important posttranscriptional modification and occurs throughout the tRNA structure.m1A at position 58 (m1A58) is the predominant m1A modification of cytoplasmic tRNA in eukaryotic cells. The formation of m1A58 on cytoplasmic tRNAs is mediated by the methyltransferase complex TRMT6/TRMT61A, in which TRMT61A functions as the catalytic subunit and TRMT6 is responsible for tRNA binding.

实验方案

Product Specific Protocols
WB protocol for TRMT61A antibody 31169-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols