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验证数据展示

  • WB analysis using 16727-1-AP (same clone as 16727-1-PBS)

    WB analysis using 16727-1-AP (same clone as 16727-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16727-1-AP (TRMT6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16727-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 16727-1-AP (same clone as 16727-1-PBS)

    IHC staining of human colon cancer using 16727-1-AP (same clone as 16727-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16727-1-AP (TRMT6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16727-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TRMT6 antibody included
  • IHC staining of human colon cancer using 16727-1-AP (same clone as 16727-1-PBS)

    IHC staining of human colon cancer using 16727-1-AP (same clone as 16727-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16727-1-AP (TRMT6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16727-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TRMT6 antibody included
  • IP experiment of HeLa using 16727-1-AP (same clone as 16727-1-PBS)

    IP experiment of HeLa using 16727-1-AP (same clone as 16727-1-PBS)

    IP result of anti-TRMT6 (IP:16727-1-AP, 4ug; Detection:16727-1-AP 1:700) with HeLa cells lysate 2000 ug. This data was developed using the same antibody clone with 16727-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 16727-1-AP (same clone as 16727-1-PBS)

    WB analysis of HepG2 using 16727-1-AP (same clone as 16727-1-PBS)

    WB result of TRMT6 antibody (16727-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRMT6 transfected HepG2 cells. This data was developed using the same antibody clone with 16727-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TRMT6 Polyclonal antibody, PBS Only

TRMT6 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 16727-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6, TRMT 6, TRM6, mRNA methyladenosine-N(1)-methyltransferase non-catalytic subunit TRM6, CGI-09

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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This antibody works well in ProteinSimple system.

Planning an IHC experiment? We recommend our IHCeasy TRMT6 Ready-To-Use IHC Kit. TRMT6 primary antibody included.

产品信息

16727-1-PBS targets TRMT6 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10176

Product name: Recombinant human TRMT6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-497 aa of BC001262

Sequence: MEGSGEQPGPQPQHPGDHRIRDGDFVVLKREDVFKAVQVQRRKKVTFEKQWFYLDNVIGHSYGTAFEVTSGGSLQPKKKREEPTAETKEAGTDNRNIVDDGKSQKLTQDDIKALKDKGIKGEEIVQQLIENSTTFRDKTEFAQDKYIKKKKKKYEAIITVVKPSTRILSIMYYAREPGKINHMRYDTLAQMLTLGNIRAGNKMIVMETCAGLVLGAMMERMGGFGSIIQLYPGGGPVRAATACFGFPKSFLSGLYEFPLNKVDSLLHGTFSAKMLSSEPKDSALVEESNGTLEEKQASEQENEDSMAEAPESNHPEDQETMETISQDPEHKGPKERGSKKDYIQEKQRRQEEQRKRHLEAAALLSERNADGLIVASRFHPTPLLLSLLDFVAPSRPFVVYCQYKEPLLECYTKLRERGGVINLRLSETWLRNYQVLPDRSHPKLLMSGGGGYLLSGFTVAMDNLKADTSLKSNASTLESHETEEPAAKKRKCPESDS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tRNA methyltransferase 6 homolog (S. cerevisiae)
别名 tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6, TRMT 6, TRM6, mRNA methyladenosine-N(1)-methyltransferase non-catalytic subunit TRM6, CGI-09
计算分子量 56 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC001262
基因名称 TRMT6
Gene ID (NCBI) 51605
RRIDAB_2878306
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UJA5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Transfer RNA (tRNA) modifications help regulate the efficiency of mRNA translation by maintaining the correct reading frames within mRNA. TRMT6 (tRNA methyltransferase 6 ), also known as GCD10 or TRM6, is a 497 amino acid protein that localizes to the nucleus and exists as a heterodimer with TRM61. Expressed in liver, brain, ovary and testis, TRMT6 functions as a substrate-binding subunit of tRNA and is thought to catalyze the formation of N(1)-methyladenine at position 58 in initiator methionyl-tRNA. TRMT6 exists as three alternatively spliced isoforms that, in response to DNA damage, may be phosphorylated by ATM or ATR. This antibody is specific to 56kd TRMT6 protein.

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