验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, mouse testis tissue, NIH/3T3 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
产品信息
18255-1-AP targets TRMT5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag13075 Product name: Recombinant human TRMT5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 161-509 aa of BC108284 Sequence: QLNVSPQISKYNLELTYEHFKSEEILRAVLPEGQDVTSGFSRIGHIAHLNLRDHQLPFKHLIGQVMIDKNPGITSAVNKINNIDNMYRNFQMEVLSGEQNMMTKVRENNYTYEFDFSKVYWNPRLSTEHSRITELLKPGDVLFDVFAGVGPFAIPVAKKNCTVFANDLNPESHKWLLYNCKLNKVDQKVKVFNLDGKDFLQGPVKEELMQLLGLSKERKPSVHVVMNLPAKAIEFLSAFKWLLDGQPCSSEFLPIVHCYSFSKDANPAEDVRQRAGAVLGISLEACSSVHLVRNVAPNKEMLCITFQIPASVLYKNQTRNPENHEDPPLKRQRTAEAFSDEKTQIVSNT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae) |
| 别名 | KIAA1393, M1G methyltransferase, TRM5, TRMT5, tRNA [GM37] methyltransferase, tRNA methyltransferase 5 |
| 计算分子量 | 509 aa, 58 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | BC108284 |
| 基因名称 | TRMT5 |
| Gene ID (NCBI) | 57570 |
| RRID | AB_2209625 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q32P41 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRMT5 antibody 18255-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Mol Biosci Integrated Strategies of Diverse Feature Selection Methods Identify Aging-Based Reliable Gene Signatures for Ischemic Cardiomyopathy. | ||
J Zhejiang Univ Sci B Targeting TRMT5 suppresses hepatocellular carcinoma progression via inhibiting the HIF-1α pathways
| ||
Cell PAX translocations remodel mitochondrial metabolism through altered leucine usage in rhabdomyosarcoma |
