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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 85897-5-RR (same clone as 85897-5-PBS)

    WB analysis using 85897-5-RR (same clone as 85897-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85897-5-RR (TRMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85897-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 85897-5-RR (same clone as 85897-5-PBS)

    IF Staining of HeLa using 85897-5-RR (same clone as 85897-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRMT1 antibody (85897-5-RR, Clone: 250178D5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85897-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85897-5-RR

    Affinity and Kinetic Characterization of 85897-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85897-5-RR against Human TRMT1 were performed. The affinity constant is 26.2 pM.

TRMT1 Recombinant antibody, PBS Only

TRMT1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85897-5-PBS

产品说明书

CloneNo. 250178D5

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, Indirect ELISA

别名

EC:2.1.1.216, TRM1, TRMT 1, tRNA (guanine(26)-N(2))-dimethyltransferase, tRNA 2,2-dimethylguanosine-26 methyltransferase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85897-5-PBS targets TRMT1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 TRMT1 fusion protein Ag6837 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae)
别名 EC:2.1.1.216, TRM1, TRMT 1, tRNA (guanine(26)-N(2))-dimethyltransferase, tRNA 2,2-dimethylguanosine-26 methyltransferase
计算分子量 81 kDa
观测分子量75 kDa
GenBank蛋白编号BC002492
基因名称 TRMT1
Gene ID (NCBI) 55621
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NXH9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRMT1 is an RNA methyltransferase responsible for the formation of N2,N2-dimethylguanosine (m2,2G) at position 26 in both cytosolic and mitochondrial tRNAs.TRMT1-catalyzed tRNA modifications are important for maintenance of global protein translation levels including proteins involved in cellular growth, development, and stress response. Human neural stem cells with TRMT1 knockdowns were found to have hypersensitivity to redox stress, implicating TRMT1 and the m2,2G26 modification in the regulation of redox homeostasis.