验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, COLO 320 cells, HeLa cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12324-1-AP targets TRIP4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2987 Product name: Recombinant human TRIP4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 281-581 aa of BC012448 Sequence: QVIDDESDYFASDSNQWLSKLERETLQKREEELRELRHASRLSKKVTIDFAGRKILEEENSLAEYHSRLDETIQAIANGTLNQPLTKLDRSSEEPLGVLVNPNMYQSPPQWVDHTGAASQKKAFRSSGFGLEFNSFQHQLRIQDQEFQEGFDGGWCLSVHQPWASLLVRGIKRVEGRSWYTPHRGRLWIAATAKKPSPQEVSELQATYRLLRGKDVEFPNDYPSGCLLGCVDLIDCLSQKQFKEQFPDISQESDSPFVFICKNPQEMVVKFPIKGNPKIWKLDSKIHQGAKKGLMKQNKAV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | thyroid hormone receptor interactor 4 |
| 别名 | ASC 1, ASC-1, HsT17391, RQT4, Thyroid receptor-interacting protein 4 |
| 计算分子量 | 581 aa, 66 kDa |
| 观测分子量 | 60-65 kDa |
| GenBank蛋白编号 | BC012448 |
| 基因名称 | TRIP4 |
| Gene ID (NCBI) | 9325 |
| RRID | AB_10646482 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15650 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Thyroid hormone receptor interactor 4(TRIP4), other named ASC1, is a transcriptional coactivator that promote transcriptional efficiencies. It harbors an autonomous transactivation domain that contains a putative zinc finger motif, which serves as a binding site for nuclear receptors for thyroid, estrogen, all-trans-retinoic acid and RXR by interaction with SRC-1 or CBP/p300. There are two forms of ASC1 have been detected in mRNA level, isoform1(581aa, ~65kd) and isoform2(539aa,~60kd)(12077347,12390891)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for TRIP4 antibody 12324-1-AP | Download protocol |
| WB protocol for TRIP4 antibody 12324-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Genet Med A pathogenic UFSP2 variant in an autosomal recessive form of pediatric neurodevelopmental anomalies and epilepsy. | ||
bioRxiv The Ski2 helicase ASCC3 unwinds DNA upon fork stalling to control replication stress responses.
| ||
Atherosclerosis The m6A demethylase FTO suppresses perivascular adipose tissue browning and exacerbates vascular injury via m6A-mediated destabilization of Irx3 mRNA and the IRX3/UCP1 axis. | ||
Exp Cell Res Molecular mechanism of MAFB transcriptional activation of PPARD in regulating adipose browning and protecting against vascular endothelial cell injury. | ||
Cell Rep Ski2-like helicase ASCC3 unwinds DNA upon fork stalling to control replication stress responses. |
