验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, MCF-7 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MDCK cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 8 publications below |
产品信息
16124-1-AP targets TRIOBP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, canine samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, canine |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag9092 Product name: Recombinant human TARA protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 5-379 aa of BC013278 Sequence: EATDSRTPEVPAGEGPRRGLGAPLTEDQQNRLSEEIEKKWQELEKLPLRENKRVPLTALLNQSRGERRGPPSDGHEALEKEVQALRAQLEAWRLQGEAPQSALRSQEDGHIPPGYISQEACERSLAEMESSHQQVMEELQRHHERELQRLQQEKEWLLAEETAATASAIEAMKKAYQEELSRELSKTRSLQQGPDGLRKQHQSDVEALKRELQVLSEQYSQKCLEIGALMRQAEEREHTLRRCQQEGQELLRHNQELHGRLSEEIDQLRGFIASQGMGNGCGRSNERSSCELEVLLRVKENELQYLKKEVQCLRDELQMMQKDKRFTSGKYQDVYVELSHIKTRSEREIEQLKEHLRLAMAALQEKESMRNSLAE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TRIO and F-actin binding protein |
| 别名 | Trio-associated repeat on actin, TRIO and F-actin-binding protein, TRF1-associated protein of 68 kDa, TARA, HRIHFB2122 |
| 计算分子量 | 2356 aa, 261 kDa |
| 观测分子量 | 68-75 kDa |
| GenBank蛋白编号 | BC013278 |
| 基因名称 | TRIOBP |
| Gene ID (NCBI) | 11078 |
| RRID | AB_2209237 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H2D6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for TRIOBP antibody 16124-1-AP | Download protocol |
| IHC protocol for TRIOBP antibody 16124-1-AP | Download protocol |
| WB protocol for TRIOBP antibody 16124-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Unbalanced bidirectional radial stiffness gradients within the organ of Corti promoted by TRIOBP. | ||
Mol Neurobiol Essential Role of Sptan1 in Cochlear Hair Cell Morphology and Function Via Focal Adhesion Signaling. | ||
J Cell Sci GIPC3 couples to MYO6 and PDZ domain proteins and shapes the hair cell apical region | ||
Cell Mol Life Sci TRIOBP modulates β-catenin signaling by regulation of miR-29b in idiopathic pulmonary fibrosis |
