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验证数据展示

  • WB analysis using 31976-1-AP

    WB analysis using 31976-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31976-1-AP (TRIML2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 31976-1-AP

    IHC staining of mouse testis using 31976-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31976-1-AP (TRIML2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TRIML2 Polyclonal antibody

TRIML2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31976-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

EC:2.3.2.27, Probable E3 ubiquitin-protein ligase TRIML2, RING-type E3 ubiquitin transferase TRIML2, SPRY domain-containing protein 6, SPRYD6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31976-1-AP targets TRIML2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 TRIML2 fusion protein Ag37106 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tripartite motif family-like 2
别名 EC:2.3.2.27, Probable E3 ubiquitin-protein ligase TRIML2, RING-type E3 ubiquitin transferase TRIML2, SPRY domain-containing protein 6, SPRYD6
计算分子量50 kDa
观测分子量50 kDa
GenBank蛋白编号BC111959
基因名称 TRIML2
Gene ID (NCBI) 205860
RRIDAB_3670164
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8N7C3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tripartite motif family like 2 (TRIML2, also known as SPRYD6) is a member of the tripartite motif (TRIM) family of proteins. It probably interacts with p53 to facilitate the modification of p53 with small ubiquitin-like modifier 2 (SUMO2) (PMID: 25256710). By regulating p53, TRIML2 may also be an important factor in the pathogenesis of Alzheimer's disease (PMID: 27471163).

实验方案

Product Specific Protocols
WB protocol for TRIML2 antibody 31976-1-APDownload protocol
IHC protocol for TRIML2 antibody 31976-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols