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Host
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验证数据展示

  • WB analysis using 67268-1-Ig (same clone as 67268-1-PBS)

    WB analysis using 67268-1-Ig (same clone as 67268-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67268-1-Ig (TRIM9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67268-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 67268-1-Ig (same clone as 67268-1-PBS)

    IHC staining of rat brain using 67268-1-Ig (same clone as 67268-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67268-1-Ig (TRIM9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67268-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 67268-1-Ig (same clone as 67268-1-PBS)

    IHC staining of rat brain using 67268-1-Ig (same clone as 67268-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67268-1-Ig (TRIM9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67268-1-PBS in a different storage buffer formulation.

TRIM9 Monoclonal antibody, PBS Only

TRIM9 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67268-1-PBS

产品说明书

CloneNo. 1D2B10

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat, Pig

应用

WB, IHC, Indirect ELISA

别名

KIAA0282, RING finger protein 91, RNF91, SPRING, TRIM9, tripartite motif containing 9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67268-1-PBS targets TRIM9 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat, Pig
免疫原 TRIM9 fusion protein Ag27856 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tripartite motif-containing 9
别名 KIAA0282, RING finger protein 91, RNF91, SPRING, TRIM9, tripartite motif containing 9
计算分子量 80 kDa
观测分子量 90 kDa, 79 kDa, 61 kDa
GenBank蛋白编号BC013414
基因名称 TRIM9
Gene ID (NCBI) 114088
RRIDAB_2882538
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9C026
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRIM9(E3 ubiquitin-protein ligase TRIM9) is also named as RNF91 and belongs to the TRIM/RBCC family. TRIM9 protein is a brain-specific E3 ubiquitin ligase. Importantly, TRIM9 is present in LBs found in DLB and PD, suggesting that TRIM9 not only plays roles in the regulation of neuronal functions, but also participates in the formation or breakdown of abnormal inclusions through its ligase activity and besides the striatum and hippocampus, the highest expression of TRIM9 protein is seen in the frontal, temporal and occipital cortices through the western blot (PMID:20085810). It has been reported that TRIM9 is highly expressed in the cerebral cortex in mouse and human brains and that TRIM9 is decreased in damaged brains in patients who have Parkinson disease and dementia with Lewy bodies(PMID:22337885). It has 3 isoforms with molecular mass of 79, 90 and 61 kDa.