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  • Featured Product
  • KD/KO Validated

TRIM8 Polyclonal antibody

TRIM8 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 27463-1-AP

Print datasheet

Synonyms

GERP, RING finger protein 27, RNF27, TRIM8, tripartite motif containing 8

验证数据展示

  • WB analysis of L02 using 27463-1-AP

    WB analysis of L02 using 27463-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 27463-1-AP (TRIM8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 27463-1-AP

    WB analysis of HepG2 using 27463-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 27463-1-AP (TRIM8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 27463-1-AP

    IHC staining of human breast cancer using 27463-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27463-1-AP (TRIM8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRIM8 antibody included
  • IHC staining of human breast cancer using 27463-1-AP

    IHC staining of human breast cancer using 27463-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27463-1-AP (TRIM8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRIM8 antibody included

经过测试的应用

Positive WB detected inHepG2 cells, L02 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TRIM8 Ready-To-Use IHC Kit. TRIM8 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27463-1-AP targets TRIM8 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IP, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen TRIM8 fusion protein Ag26517 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tripartite motif-containing 8
Synonyms GERP, RING finger protein 27, RNF27, TRIM8, tripartite motif containing 8
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC021925
Gene Symbol TRIM8
Gene ID (NCBI) 81603
RRIDAB_2880877
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZR9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TRIM8 antibody 27463-1-APDownload protocol
IHC protocol for TRIM8 antibody 27463-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF,IP

Oncogene

Mutual regulation between TRIM21 and TRIM8 via K48-linked ubiquitination

Authors - Lin Wang
  • KD Validated
View Article
humanWB

Sci Rep

Five hypoxia and immunity related genes as potential biomarkers for the prognosis of osteosarcoma.

Authors - Dachang Liu
View Article
humanWB

Exp Mol Pathol

Down-regulation of long noncoding RNA LINC00472 alleviates sepsis-induced acute hepatic injury by regulating miR-373-3p/TRIM8 axis.

Authors - Lei Li
View Article