验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, L02 cells |
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27463-1-AP targets TRIM8 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag26517 Product name: Recombinant human TRIM8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 180-351 aa of BC021925 Sequence: CDVEIRRNEIRKMLMKQQDRLEEREQDIEDQLYKLESDKRLVEEKVNQLKEEVRLQYEKLHQLLDEDLRQTVEVLDKAQAKFCSENAAQALHLGERMQEAKKLLGSLQLLFDKTEDVSFMKNTKSVKILMDRTQTCTSSSLSPTKIGHLNSKLFLNEVAKKEKQLRKMLEGP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tripartite motif-containing 8 |
| 别名 | RNF27, RING-type E3 ubiquitin transferase TRIM8, Glioblastoma-expressed RING finger protein, GERP, EC:2.3.2.27 |
| 计算分子量 | 61 kDa |
| 观测分子量 | 61 kDa |
| GenBank蛋白编号 | BC021925 |
| 基因名称 | TRIM8 |
| Gene ID (NCBI) | 81603 |
| RRID | AB_2880877 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BZR9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRIM8 (Tripartite motif containing protein 8), also known as RNF27 (RING finger protein 27), a member of TRIM family proteins, is known to play a dual role as both tumor suppressor and oncogene, and to function at the crosstalk of cancer and innate immunity. TRIM8 can function at two subcellular sites-nucleus and cytoplasm-to regulate NF-κB, one of the central signaling pathways that plays a critical role in carcinogenesis and inflammatory diseases (PMID: 33230447, 22262183). TRIM8 is composed of 551 amino acids (aa) with a molecular weight of 61.5 kDa, contains a Nuclear Localization Signal (NLS), and forms specific nuclear structures similar to the TRIM19/PML Nuclear Bodies (NBs) (PMID: 33807506, 29182544).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIM8 antibody 27463-1-AP | Download protocol |
| IHC protocol for TRIM8 antibody 27463-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncogene Mutual regulation between TRIM21 and TRIM8 via K48-linked ubiquitination
| ||
Sci Rep Five hypoxia and immunity related genes as potential biomarkers for the prognosis of osteosarcoma. | ||
Cancer Med TRIM8 promotes ovarian cancer proliferation and migration by targeting VDAC2 for ubiquitination and degradation
| ||
J Orthop Surg Res The prognostic value of ubiquitin/ubiquitin-like-related genes along with immune cell infiltration and clinicopathological features in osteosarcoma | ||
Exp Mol Pathol Down-regulation of long noncoding RNA LINC00472 alleviates sepsis-induced acute hepatic injury by regulating miR-373-3p/TRIM8 axis. | ||
Adipocyte Inhibiting TRIM8 alleviates adipocyte inflammation and insulin resistance by regulating the DUSP14/MAPKs pathway
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