验证数据展示
经过测试的应用
Positive WB detected in | mouse heart tissue, rat heart tissue |
Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
26285-1-AP targets TRIM7 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with mouse samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IHC, IF, CoIP |
经测试反应性 | mouse |
文献引用反应性 | human, mouse |
免疫原 | TRIM7 fusion protein Ag23453 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | tripartite motif-containing 7 |
别名 | RING finger protein 90, GNIP, Glycogenin-interacting protein, Glycogenin interacting protein, EC:2.3.2.27 |
观测分子量 | 50-55 kDa |
GenBank蛋白编号 | BC132863 |
基因名称 | TRIM7 |
Gene ID (NCBI) | 81786 |
RRID | AB_2880462 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9C029 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for TRIM7 antibody 26285-1-AP | Download protocol |
IHC protocol for TRIM7 antibody 26285-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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EBioMedicine N6-Methyladenosine modification of the TRIM7 positively regulates tumorigenesis and chemoresistance in osteosarcoma through ubiquitination of BRMS1. | ||
J Virol Nascent Transcriptomics Reveal Cellular Prolytic Factors Upregulated Upstream of the Latent-to-Lytic Switch Protein of Epstein-Barr Virus.
| ||
Vet Microbiol TRIM7 inhibits encephalomyocarditis virus replication by activating interferon-β signaling pathway | ||
Sci Rep The E3 ligase TRIM7 suppresses the tumorigenesis of gastric cancer by targeting SLC7A11
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mBio The E3 ligase TRIM22 restricts SARS-CoV-2 replication by promoting proteasomal degradation of NSP8 |