验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, rat heart tissue |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26285-1-AP targets TRIM7 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag23453 Product name: Recombinant human TRIM7 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 435-511 aa of BC132863 Sequence: LQLNGGQYWAVTSPERSPLSCGHLSRVRVALDLEVGAVSFYAVEDMRHLYTFRVNFQERVFPLFSVCSTGTYLRIWP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tripartite motif-containing 7 |
| 别名 | RING finger protein 90, GNIP, Glycogenin-interacting protein, Glycogenin interacting protein, EC:2.3.2.27 |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | BC132863 |
| 基因名称 | TRIM7 |
| Gene ID (NCBI) | 81786 |
| RRID | AB_2880462 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9C029 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIM7 antibody 26285-1-AP | Download protocol |
| IHC protocol for TRIM7 antibody 26285-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EBioMedicine N6-Methyladenosine modification of the TRIM7 positively regulates tumorigenesis and chemoresistance in osteosarcoma through ubiquitination of BRMS1. | ||
J Virol Nascent Transcriptomics Reveal Cellular Prolytic Factors Upregulated Upstream of the Latent-to-Lytic Switch Protein of Epstein-Barr Virus.
| ||
Vet Microbiol TRIM7 inhibits encephalomyocarditis virus replication by activating interferon-β signaling pathway | ||
Sci Rep The E3 ligase TRIM7 suppresses the tumorigenesis of gastric cancer by targeting SLC7A11
| ||
mBio The E3 ligase TRIM22 restricts SARS-CoV-2 replication by promoting proteasomal degradation of NSP8 | ||
Am J Pathol E3 Ubiquitin Ligase TRIM7 Alleviates LPS-Induced Acute Lung Injury via Inhibiting NLRP3 Inflammasome Activation |