验证数据展示
产品信息
31990-1-PBS targets TRIM65 in WB, Indirect ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, Indirect ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag34639 Product name: Recombinant human TRIM65 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 320-517 aa of NM_173547 Sequence: CPLRRKLWQNYRNLTFDPVSANRHFYLSRQDQQVKHCRQSRGPGGPGSFELWQVQCAQSFQAGHHYWEVRASDHSVTLGVSYPQLPRCRLGPHTDNIGRGPCSWGLCVQEDSLQAWHNGEAQRLPGVSGRLLGMDLDLASGCLTFYSLEPQTQPLYTFHALFNQPLTPVFWLLEGRTLTLCHQPGAVFPLGPQEEVLS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tripartite motif-containing 65 |
| 别名 | E3 ubiquitin-protein ligase TRIM65, EC:2.3.2.27, Tripartite motif-containing protein 65 |
| 计算分子量 | 57 kDa |
| 观测分子量 | 57 kDa, 70 kDa |
| GenBank蛋白编号 | NM_173547 |
| 基因名称 | TRIM65 |
| Gene ID (NCBI) | 201292 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q6PJ69 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Tripartite motif-containing protein 65 (TRIM65) is a member of the TRIM family of RING-type E3 ubiquitin ligases. It contains a RING-finger domain, a B-box domain, a coiled-coil domain, and a SPRY domain (PMID: 40473847). As an E3 ubiquitin ligase, TRIM65 is involved in the ubiquitination of substrate proteins, playing a role in various intracellular physiological processes, including intracellular signaling, protein degradation, cell proliferation, apoptosis, carcinogenesis, autophagy, and phenotypic transformation (PMID: 38939997). TRIM65 has been shown to promote ubiquitination and degradation of several proteins, such as TOX4, which is involved in apoptosis, and ARHGAP35, which is involved in cell migration (PMID: 38212319; 31332286). The 70 kDa band might be the result of SUMO modification.
