×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis of MCF-7 using 15256-1-AP (same clone as 15256-1-PBS)

    WB analysis of MCF-7 using 15256-1-AP (same clone as 15256-1-PBS)

    WB result of TRIM62 antibody (15256-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM62 transfected MCF-7 cells. This data was developed using the same antibody clone with 15256-1-PBS in a different storage buffer formulation.

  • WB analysis using 15256-1-AP (same clone as 15256-1-PBS)

    WB analysis using 15256-1-AP (same clone as 15256-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15256-1-AP (TRIM62 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15256-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 15256-1-AP (same clone as 15256-1-PBS)

    IHC staining of mouse liver using 15256-1-AP (same clone as 15256-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15256-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 15256-1-AP (same clone as 15256-1-PBS)

    IHC staining of mouse liver using 15256-1-AP (same clone as 15256-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15256-1-PBS in a different storage buffer formulation.

  • IHC staining of rat ovary using 15256-1-AP (same clone as 15256-1-PBS)

    IHC staining of rat ovary using 15256-1-AP (same clone as 15256-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15256-1-PBS in a different storage buffer formulation.

  • IHC staining of rat ovary using 15256-1-AP (same clone as 15256-1-PBS)

    IHC staining of rat ovary using 15256-1-AP (same clone as 15256-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15256-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 15256-1-AP (same clone as 15256-1-PBS)

    IF Staining of U2OS using 15256-1-AP (same clone as 15256-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using TRIM62 antibody (15256-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 15256-1-PBS in a different storage buffer formulation.

TRIM62 Polyclonal antibody, PBS Only

TRIM62 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 15256-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

E3 ubiquitin-protein ligase TRIM62, EC:2.3.2.27, tripartite motif containing 62, Tripartite motif-containing protein 62

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

15256-1-PBS targets TRIM62 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7143

Product name: Recombinant human TRIM62 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 126-475 aa of BC001222

Sequence: TGIDDAFDELQRELKDQLQALQDSEREHTEALQLLKRQLAETKSSTKSLRTTIGEAFERLHRLLRERQKAMLEELEADTARTLTDIEQKVQRYSQQLRKVQEGAQILQERLAETDRHTFLAGVASLSERLKGKIHETNLTYEDFPTSKYTGPLQYTIWKSLFQDIHPVPAALTLDPGTAHQRLILSDDCTIVAYGNLHPQPLQDSPKRFDVEVSVLGSEAFSSGVHYWEVVVAEKTQWVIGLAHEAASRKGSIQIQPSRGFYCIVMHDGNQYSACTEPWTRLNVRDKLDKVGVFLDYDQGLLIFYNADDMSWLYTFREKFPGKLCSYFSPGQSHANGKNVQPLRINTVRI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tripartite motif-containing 62
别名 E3 ubiquitin-protein ligase TRIM62, EC:2.3.2.27, tripartite motif containing 62, Tripartite motif-containing protein 62
计算分子量 54 kDa
观测分子量54 kDa
GenBank蛋白编号BC001222
基因名称 TRIM62
Gene ID (NCBI) 55223
RRIDAB_3669202
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BVG3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRIM62(Tripartite motif-containing protein 62) belongs to the TRIM/RBCC family. TRIM proteins are involved in a broad range of biological processes, including some that underlie genetic disorders, neurological disorders and cancers. TRIM62 is shown to promote the release of HIV from infected cells, and a TRIM62 protein that has a mutation in its RING domain was more active than the native protein in promoting HIV release(PMID:18836477).

||
New chat

Able™

正在加载，请稍候...