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验证数据展示

  • IHC staining of mouse liver using 24371-1-AP (same clone as 24371-1-PBS)

    IHC staining of mouse liver using 24371-1-AP (same clone as 24371-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24371-1-AP (TRIM61 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24371-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 24371-1-AP (same clone as 24371-1-PBS)

    IF Staining of U2OS using 24371-1-AP (same clone as 24371-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using TRIM61 antibody (24371-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 24371-1-PBS in a different storage buffer formulation.

TRIM61 Polyclonal antibody, PBS Only

TRIM61 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 24371-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, Indirect ELISA

别名

tripartite motif containing 61, TRIM, RNF35, RING finger protein 35

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

24371-1-PBS targets TRIM61 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag21656

Product name: Recombinant human TRIM61 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 176-209 aa of BC089393

Sequence: MESPSVTRLECSCTISAHFNLRLPGSSDSSASGS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tripartite motif-containing 61
别名 tripartite motif containing 61, TRIM, RNF35, RING finger protein 35
计算分子量 209 aa, 24 kDa
GenBank蛋白编号BC089393
基因名称 TRIM61
Gene ID (NCBI) 391712
RRIDAB_3669434
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5EBN2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRIM61 is a member of the tripartite motif (TRIM) protein family. The RING domain in TRIM proteins, including TRIM61, can mediate the conjugation of proteins with ubiquitin, small ubiquitin-like modifier (SUMO), or the ubiquitin-like molecule IFN-stimulated protein of 15 kDa (ISG15), contributing to the biological flexibility of TRIM proteins. The E3 ligase activity of TRIM proteins, such as TRIM61, is essential for their role in post-translational modification, which plays a crucial role in cellular processes often resulting in targeted degradation of conjugated proteins.

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