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TRIM54 Polyclonal antibody

TRIM54 Polyclonal Antibody for WB, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IHC, ELISA

Conjugate

Unconjugated

Cat No : 21074-1-AP

Print datasheet

Synonyms

MURF, MURF 3, MuRF3, RING finger protein 30, RNF30, TRIM54, tripartite motif containing 54

验证数据展示

  • WB analysis of mouse heart using 21074-1-AP

    WB analysis of mouse heart using 21074-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 21074-1-AP (TRIM54 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 21074-1-AP

    IHC staining of human heart using 21074-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart slide using 21074-1-AP (TRIM54 Antibody) at dilution of 1:50.

  • IHC staining of human heart using 21074-1-AP

    IHC staining of human heart using 21074-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart slide using 21074-1-AP (TRIM54 Antibody) at dilution of 1:50.

  • IF Staining of HepG2 using 21074-1-AP

    IF Staining of HepG2 using 21074-1-AP

    Immunofluorescent analysis of HepG2 cells, using TRIM54 antibody 21074-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • WB analysis of human heart using 21074-1-AP

    WB analysis of human heart using 21074-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 21074-1-AP (TRIM54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse heart tissue, human skeletal muscle tissue, human heart tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

21074-1-AP targets TRIM54 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IHC, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen TRIM54 fusion protein Ag15229 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tripartite motif-containing 54
Synonyms MURF, MURF 3, MuRF3, RING finger protein 30, RNF30, TRIM54, tripartite motif containing 54
Calculated Molecular Weight 358 aa, 40 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC141807
Gene Symbol TRIM54
Gene ID (NCBI) 57159
RRIDAB_10694832
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYV2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM54 (Tripartite motif-containing protein 54) is also names as MURF, MURF3, RNF30. The expression of TRIM54 is required for skeletal myoblast differentiation and myotube fusion. It is expressed in heart and skeletal muscle specifically (PMID:11243782). It has two isoforms with the molecular weight of 40KDa and 45KDa. It can be dimerizated and hetero-dimerizated to form homooligomer and heterooligomer (PMID:15967462).

实验方案

Product Specific Protocols
WB protocol for TRIM54 antibody 21074-1-APDownload protocol
IHC protocol for TRIM54 antibody 21074-1-APDownload protocol
IF protocol for TRIM54 antibody 21074-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Oncol

Targeting TRIM54/Axin1/β-Catenin Axis Prohibits Proliferation and Metastasis in Hepatocellular Carcinoma.

Authors - Jinrong Zhu
View Article
humanWB

J Cell Mol Med

NT5DC2 promotes leiomyosarcoma tumour cell growth via stabilizing unpalmitoylated TEAD4 and generating a positive feedback loop.

Authors - Bowen Hu
View Article
humanWB

Hepatol Commun

Sorafenib induces ferroptosis by promoting TRIM54-mediated FSP1 ubiquitination and degradation in hepatocellular carcinoma

Authors - Man-Ru Liu
View Article