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验证数据展示

  • FC experiment of HeLa using 83614-5-RR (same clone as 83614-5-PBS)

    FC experiment of HeLa using 83614-5-RR (same clone as 83614-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug TRIM46 Recombinant antibody (83614-5-RR, Clone:240557C2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83614-5-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83614-5-RR (same clone as 83614-5-PBS)

    IF Staining of HepG2 using 83614-5-RR (same clone as 83614-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TRIM46 antibody (83614-5-RR, Clone: 240557C2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83614-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83614-5-RR

    Affinity and Kinetic Characterization of 83614-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83614-5-RR against Human TRIM46 were performed. The affinity constant is 1.48 nM.

TRIM46 Recombinant monoclonal antibody, PBS Only

TRIM46 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83614-5-PBS

产品说明书

CloneNo. 240557C2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

240557C2, GeneY, TRIFIC, tripartite motif containing 46, Tripartite motif-containing protein 46

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83614-5-PBS targets TRIM46 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag15290

Product name: Recombinant human TRIM46 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC069416

Sequence: MAEGEDMQTFTSIMDALVRISTSMKNMEKELLCPVCQEMYKQPLVLPCTHNVCQACAREVLGQQGYIGHGGDPSSEPTSPASTPSTRSPRLSRRTLPKPDRLDRLLKSGFGTYPGRKRGALHPQVIMFPCPACQGDVELGERGLAGLFRNLTLERVVERYRQSVSVGGAILCQLCKPPPLEATKGCTECRATFCNECFKLFHPWGTQKAQHEPTLPTLSFRPKGLMCPDHKEEVTHYCKTCQRLVCQLCRVRRTHSGHKITPVLSAYQALKDKLTKSLTYILGNQDTVQTQICELEEAVRHTEVSGQQAKEEVSQLVRGLGAVLEEKRASLLQAIEECQQERLARLSAQI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tripartite motif-containing 46
别名 240557C2, GeneY, TRIFIC, tripartite motif containing 46, Tripartite motif-containing protein 46
计算分子量 759 aa, 83 kDa
GenBank蛋白编号BC069416
基因名称 TRIM46
Gene ID (NCBI) 80128
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ7Z4K8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tripartite motif-containing protein 46 (TRIM46), a microtubule-associated protein, is involved in the formation of parallel microtubule bundles linked by cross-bridges in the proximal axon. Required for the uniform orientation and maintenance of the parallel microtubule fascicles, which are important for efficient cargo delivery and trafficking in axons. Thereby also required for proper axon specification, the establishment of neuronal polarity, and proper neuronal migration. The TRIM46 protein reported in previous studies includes the long isoform TRIM46L (83 kDa) and the short isoform TRIM46S (60 kDa).

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