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验证数据展示

  • WB analysis using 83614-2-RR

    WB analysis using 83614-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83614-2-RR (TRIM46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of U-87 MG using 83614-2-RR

    WB analysis of U-87 MG using 83614-2-RR

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 83614-2-RR (TRIM46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 83614-2-RR

    IHC staining of mouse brain using 83614-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83614-2-RR (TRIM46 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 83614-2-RR

    IHC staining of mouse brain using 83614-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83614-2-RR (TRIM46 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 83614-2-RR

    IF Staining of mouse brain using 83614-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TRIM46 antibody (83614-2-RR, Clone: 240557E1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 83614-2-RR

    IF Staining of A549 using 83614-2-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TRIM46 antibody (83614-2-RR, Clone: 240557E1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • FC experiment of HeLa using 83614-2-RR

    FC experiment of HeLa using 83614-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TRIM46 Recombinant antibody (83614-2-RR, Clone:240557E1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83614-2-RR

    Affinity and Kinetic Characterization of 83614-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83614-2-RR against Human TRIM46 were performed. The affinity constant is below 1 pM.

TRIM46 Recombinant antibody

TRIM46 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Cat No. 83614-2-RR

产品说明书

CloneNo. 240557E1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

别名

Tripartite, fibronectin type-III and C-terminal SPRY motif protein, Tripartite motif-containing protein 46, tripartite motif containing 46, TRIFIC, GeneY

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, U-87 MG cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83614-2-RR targets TRIM46 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TRIM46 fusion protein Ag15290 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tripartite motif-containing 46
别名 Tripartite, fibronectin type-III and C-terminal SPRY motif protein, Tripartite motif-containing protein 46, tripartite motif containing 46, TRIFIC, GeneY
计算分子量 759 aa, 83 kDa
观测分子量83 kDa
GenBank蛋白编号BC069416
基因名称 TRIM46
Gene ID (NCBI) 80128
RRIDAB_3671227
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ7Z4K8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tripartite motif-containing protein 46 (TRIM46), a microtubule-associated protein, is involved in the formation of parallel microtubule bundles linked by cross-bridges in the proximal axon. Required for the uniform orientation and maintenance of the parallel microtubule fascicles, which are important for efficient cargo delivery and trafficking in axons. Thereby also required for proper axon specification, the establishment of neuronal polarity, and proper neuronal migration. The TRIM46 protein reported in previous studies includes the long isoform TRIM46L (83 kDa) and the short isoform TRIM46S (60 kDa).

实验方案

Product Specific Protocols
WB protocol for TRIM46 antibody 83614-2-RRDownload protocol
IHC protocol for TRIM46 antibody 83614-2-RRDownload protocol
IF protocol for TRIM46 antibody 83614-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols