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验证数据展示

  • WB analysis using 21026-1-AP (same clone as 21026-1-PBS)

    WB analysis using 21026-1-AP (same clone as 21026-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21026-1-AP (TRIM46 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21026-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 21026-1-AP (same clone as 21026-1-PBS)

    IF Staining of mouse brain using 21026-1-AP (same clone as 21026-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TRIM46 antibody (21026-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21026-1-PBS in a different storage buffer formulation.

  • WB analysis using 21026-1-AP (same clone as 21026-1-PBS)

    WB analysis using 21026-1-AP (same clone as 21026-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21026-1-AP (TRIM46 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21026-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 21026-1-AP (same clone as 21026-1-PBS)

    IF Staining of mouse brain using 21026-1-AP (same clone as 21026-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TRIM46 antibody (21026-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21026-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 21026-1-AP (same clone as 21026-1-PBS)

    IF Staining of A549 using 21026-1-AP (same clone as 21026-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TRIM46 antibody (21026-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 21026-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TRIM46 Polyclonal antibody, PBS Only

TRIM46 Polyclonal Antibody for WB, IF/ICC, IF-P, Indirect ELISA
Cat No. 21026-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IF-P, Indirect ELISA

别名

GeneY, TRIFIC, tripartite motif containing 46, Tripartite motif-containing protein 46, Tripartite, fibronectin type-III and C-terminal SPRY motif protein

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

21026-1-PBS targets TRIM46 in WB, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag15290

Product name: Recombinant human TRIM46 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC069416

Sequence: MAEGEDMQTFTSIMDALVRISTSMKNMEKELLCPVCQEMYKQPLVLPCTHNVCQACAREVLGQQGYIGHGGDPSSEPTSPASTPSTRSPRLSRRTLPKPDRLDRLLKSGFGTYPGRKRGALHPQVIMFPCPACQGDVELGERGLAGLFRNLTLERVVERYRQSVSVGGAILCQLCKPPPLEATKGCTECRATFCNECFKLFHPWGTQKAQHEPTLPTLSFRPKGLMCPDHKEEVTHYCKTCQRLVCQLCRVRRTHSGHKITPVLSAYQALKDKLTKSLTYILGNQDTVQTQICELEEAVRHTEVSGQQAKEEVSQLVRGLGAVLEEKRASLLQAIEECQQERLARLSAQI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tripartite motif-containing 46
别名 GeneY, TRIFIC, tripartite motif containing 46, Tripartite motif-containing protein 46, Tripartite, fibronectin type-III and C-terminal SPRY motif protein
计算分子量 759 aa, 83 kDa
观测分子量83 kDa
GenBank蛋白编号BC069416
基因名称 TRIM46
Gene ID (NCBI) 80128
RRIDAB_10732843
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z4K8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tripartite motif-containing protein 46 (TRIM46), a microtubule-associated protein, is involved in the formation of parallel microtubule bundles linked by cross-bridges in the proximal axon. Required for the uniform orientation and maintenance of the parallel microtubule fascicles, which are important for efficient cargo delivery and trafficking in axons. Thereby also required for proper axon specification, the establishment of neuronal polarity, and proper neuronal migration. The TRIM46 protein reported in previous studies includes the long isoform TRIM46L (83 kDa) and the short isoform TRIM46S (60 kDa).

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