验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66896-1-Ig targets TRIM45 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag28300 Product name: Recombinant human TRIM45 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 210-562 aa of BC034943 Sequence: VCQDCVVGEHREHPCDFTSNVIHKHGDSVWELLKGTQPHVEALEEALAQIHIINSALQKRVEAVAADVRTFSEGYIKAIEEHRDKLLKQLEDIRAQKENSLQLQKAQLEQLLADMRTGVEFTEHLLTSGSDLEILITKRVVVERLRKLNKVQYSTRPGVNDKIRFCPQEKAGQCRGYEIYDLHRAREKQTASFTLLCKDAAGEIMGRGGDNVQVAVVPKDKKDSPVRTMVQDNKDGTYYISYTPKEPGVYTVWVCIKEQHVQGSPFTVTVRRKHRPHSGVFHCCTFCSSGGQKTARCACGGTMPGGYLGCGHGHKGHPGHPHWSCCGKFNEKSECTWTGGQSAPRSLLRTVAL 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | tripartite motif-containing 45 |
| 别名 | FLJ13181, RING finger protein 99, RNF99, TRIM45, tripartite motif containing 45 |
| 计算分子量 | 562aa,62 kDa; 580aa,64 kDa |
| 观测分子量 | 62-64 kDa |
| GenBank蛋白编号 | BC034943 |
| 基因名称 | TRIM45 |
| Gene ID (NCBI) | 80263 |
| RRID | AB_2882225 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9H8W5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIM45 antibody 66896-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
