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TRIM44 Monoclonal antibody

TRIM44 Monoclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

1B12H12

Cat No : 66249-1-Ig

Print datasheet

Synonyms

DIPB, HSA249128, MC7, Protein DIPB, TRIM44, tripartite motif containing 44

验证数据展示

  • WB analysis of MCF-7 using 66249-1-Ig

    WB analysis of MCF-7 using 66249-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66249-1-Ig (TRIM44 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 66249-1-Ig

    IF Staining of MCF-7 using 66249-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed MCF-7 cells using 66249-1-Ig(TRIM44 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inMCF-7 cells, human testis tissue
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

66249-1-Ig targets TRIM44 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen TRIM44 fusion protein Ag18771 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name tripartite motif-containing 44
Synonyms DIPB, HSA249128, MC7, Protein DIPB, TRIM44, tripartite motif containing 44
Calculated Molecular Weight 344 aa, 38 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC024031
Gene Symbol TRIM44
Gene ID (NCBI) 54765
RRIDAB_2881637
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96DX7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM44 is one of the family member of TRIM protein, which contains an N-terminal ubiquitin hydrolase-type zinc-finger domain, followed by a coiled-coil domain, a zinc-finger B-box homology domain, and a second coiled-coil domain near the C-terminus. Members of the TRIM protein family are involved in various cellular processes, such as cell proliferation, oncogenesis and antiviral defense.

实验方案

Product Specific Protocols
WB protocol for TRIM44 antibody 66249-1-IgDownload protocol
IF protocol for TRIM44 antibody 66249-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Immunol

Inducible TAP1 Negatively Regulates the Antiviral Innate Immune Response by Targeting the TAK1 Complex.

Authors - Zhangchuan Xia
View Article
humanWB

J Immunol

NAC1 Potentiates Cellular Antiviral Signaling by Bridging MAVS and TBK1.

Authors - Zhangchuan Xia
View Article