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TRIM40 Monoclonal antibody

TRIM40 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig and More (1)

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

1C5D3

Cat No : 67073-1-Ig

Print datasheet

Synonyms

RING finger protein 35, RNF35, TRIM40, tripartite motif containing 40

验证数据展示

  • WB analysis using 67073-1-Ig

    WB analysis using 67073-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67073-1-Ig (TRIM40 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 67073-1-Ig

    IHC staining of human small intestine using 67073-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67073-1-Ig (TRIM40 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human small intestine using 67073-1-Ig

    IHC staining of human small intestine using 67073-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67073-1-Ig (TRIM40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inNCCIT cell, THP-1 cells, pig small intestine tissue, HT-29 cells, NCCIT cells, JAR cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:600-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67073-1-Ig targets TRIM40 in WB, IHC, ELISA applications and shows reactivity with Human, Pig samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity Human, Pig
Cited Reactivityhuman
Immunogen TRIM40 fusion protein Ag21660 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tripartite motif-containing 40
Synonyms RING finger protein 35, RNF35, TRIM40, tripartite motif containing 40
Calculated Molecular Weight 258 aa, 29 kDa
Observed Molecular Weight 26-36 kDa
GenBank Accession NumberBC060785
Gene Symbol TRIM40
Gene ID (NCBI) 135644
RRIDAB_2882382
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ6P9F5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TRIM40 antibody 67073-1-IgDownload protocol
IHC protocol for TRIM40 antibody 67073-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Rep

Type I interferon/STAT1 signaling regulates UBE2M-mediated antiviral innate immunity in a negative feedback manner

Authors - Xianghui Kong
View Article