验证数据展示
经过测试的应用
| Positive WB detected in | human liver tissue, HEK-293 cells, human colon tissue |
| Positive IHC detected in | human liver tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:300-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13405-1-AP targets TRIM38 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | TRIM38 fusion protein Ag3840 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tripartite motif-containing 38 |
| 别名 | TRIM, RORET, RNF15, RING finger protein 15, EC:2.3.2.27 |
| 计算分子量 | 465 aa, 53 kDa |
| 观测分子量 | 53 kDa |
| GenBank蛋白编号 | BC026930 |
| 基因名称 | TRIM38 |
| Gene ID (NCBI) | 10475 |
| RRID | AB_2208307 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00635 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The proinflammatory cytokines TNFα and IL-1β play central roles in many diseases, including infectious diseases, autoimmunity, and cancers. TRIM38 belongs to the TRIM protein family that is involved in various biological processes, including cell proliferation and apoptosis, innate immunity, and inflammatory response. It has been shown that TRIM38 acts as a suppressor in TOLL-like receptor (TLR)-mediated IFN-β induction by promoting degradation of TRAF6 and NAP1 through the ubiquitinproteasome system. (PMID:24434549)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIM38 antibody 13405-1-AP | Download protocol |
| IHC protocol for TRIM38 antibody 13405-1-AP | Download protocol |
| IF protocol for TRIM38 antibody 13405-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) Tertiary lymphoid structure stratifies glioma into three distinct tumor subtypes. | ||
Int Immunopharmacol TRIM38 protects chondrocytes from IL-1β-induced apoptosis and degeneration via negatively modulating nuclear factor (NF)-κB signaling.
| ||
J Lipid Res Tripartite motif 38 alleviates the pathological process of NAFLD/NASH by promoting TAB2 degradation | ||
Phytomedicine Active fraction of Polyrhachis vicina (Rogers) inhibits osteoclastogenesis by targeting Trim38 mediated proteasomal degradation of TRAF6 | ||
Viruses TRIM38 Inhibits Zika Virus by Upregulating RIG-I/MDA5 Pathway and Promoting Ubiquitin-Mediated Degradation of Viral NS3 Protein |
