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- KD/KO Validated
TRIM37 Polyclonal antibody
TRIM37 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, MCF-7 cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse testis tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells, U-87 MG cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13037-1-AP targets TRIM37 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IP, IF, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | TRIM37 fusion protein Ag3690 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | tripartite motif-containing 37 |
| Synonyms | KIAA0898, MUL, Mulibrey nanism protein, POB1, TEF3, TRIM37, tripartite motif containing 37 |
| Calculated Molecular Weight | 964 aa, 108 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC036012 |
| Gene Symbol | TRIM37 |
| Gene ID (NCBI) | 4591 |
| RRID | AB_10640800 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94972 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRIM37 (tripartite motif containing 37) is a member of the tripartite motif (RING, B-Box, and coiled-coil domains) family . Its gene mutations result in mulibrey nanism, which is a rare, autosomal-recessive growth disorder of prenatal onset, with characteristic dysmorphic features, pericardial constriction, and hepatomegaly .TRIM37 is located on chromosome 17q23, encoding a protein with an estimated molecular weight of 130 kDa or 89kDa. (PMID: 28098873, PMID: 29940807, PMID: 14757854)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIM37 antibody 13037-1-AP | Download protocol |
| IHC protocol for TRIM37 antibody 13037-1-AP | Download protocol |
| IF protocol for TRIM37 antibody 13037-1-AP | Download protocol |
| IP protocol for TRIM37 antibody 13037-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Transl Oncol TRIM37 promotes gallbladder cancer proliferation by activating the Wnt/β-catenin pathway via ubiquitination of Axin1 | ||
Exp Cell Res Aerobic glycolysis and tumor progression of hepatocellular carcinoma are mediated by ubiquitin of P53 K48-linked regulated by TRIM37
| ||
Biochem Biophys Res Commun Tripartite motif containing protein 37 involves in thrombin stimulated BV-2 microglial cell apoptosis and interleukin 1β release.
| ||
Mol Cancer Res Downregulation of BASP1 promotes temozolomide resistance in gliomas via epigenetic activation of the FBXO32/NF-κB/MGMT axis | ||
Mol Med TRIM37 exacerbates hepatic ischemia/reperfusion injury by facilitating IKKγ translocation
| ||
Cell Discov The TRIM37 variants in Mulibrey nanism patients paralyze follicular helper T cell differentiation |
